PTK7 Protein, Rabbit (HEK293, His)

The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PTK7 Protein, Rabbit (HEK293, His) is 582 a.a., with molecular weight of approximately 110 kDa.

The PTK7 protein, an inactive tyrosine kinase, plays a significant role in the Wnt signaling pathway, being a component of both the non-canonical (Wnt/planar cell polarity signaling) and canonical Wnt signaling pathways. Its involvement spans diverse cellular processes, including cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 also contributes to critical developmental events, such as embryogenesis, epithelial tissue organization, and angiogenesis. Notably, it interacts with CTNNB1, underlining its participation in Wnt pathway regulation and its impact on cellular functions with broader implications in both normal and pathological contexts.

Immobilized PTK-7 at 2 μg/mL (100 μL/well) can bind Biotinylated Wnt3a protein. The ED₅₀ for this effect is 8.625 μg/mL.

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  Immobilized PTK-7 at 2 μg/mL (100 μL/well) can bind Biotinylated Wnt3a protein. The ED₅₀ for this effect is 8.625 μg/mL.

Rabbit

HEK293

C-10*His

G1SIJ6-1 (A31-T612)

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AIVFIKEPSSQDALQGRRALLRCEVEAPAPVHVYWLLNGAPVQDTERRFVQDTERRFPQVSSLSFTAVDRLQDSGAFQCVARDNVTGEEARSANASFNIKWIEGGPVVLKQPASEAEIQPQTQVTLRCHVDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPATPEHSGVYSCCAHNAFGQACSSQNFTLNIAEIEDMPPFEPRVFTAGSEERVACLPPHGLPEPSVWWEQAGVRLPTHGRVYQKGHELVFADTAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLEKPQDSQLEEGRPGYLHCLTQATPKPTVVWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTWYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWIRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPSKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPTPEESEGPGSPPPYKMIQT

approximately 110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.