PTPRD Protein, Human (HEK293, His)

PTPRD protein is a receptor-type protein tyrosine phosphatase involved in various cellular processes related to cytoskeletal proteins. It regulates cell adhesion, motility, migration, cell cycle, neuronal growth, ion channel regulation, immune response, cancer cell invasion and apoptosis. PTPRD Protein, Human (HEK293, His) is the recombinant human-derived PTPRD protein, expressed by HEK293 , with C-His labeled tag.

PKCE, a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase, plays vital roles in regulating various cellular processes associated with cytoskeletal proteins. It is involved in cell adhesion, motility, migration, cell cycle, neuron growth, ion channel regulation, immune response, cancer cell invasion, and apoptosis. PKCE mediates integrin-dependent signaling, influencing cell adhesion to the extracellular matrix, and phosphorylates proteins such as MARCKS, PTK2/FAK, vimentin (VIM), keratin-8 (KRT8), and IQGAP1 to modulate diverse cellular functions. It contributes to cell migration in response to growth factors, participates in cytokinesis, regulates myofilament function in cardiac myocytes, and is implicated in the pathogenesis of dilated cardiomyopathy. Additionally, PKCE influences neurite outgrowth, synaptic potentiation, and the immune response by phosphorylating various substrates, including GABRG2, TRPV1, CACNA1B, STAT3, TICAM2/TRAM, and NLRP5/MATER. It emerges as a multifaceted kinase with wide-ranging impacts on cellular physiology and pathology.

Human

HEK293

C-His

P23468-1 (E21-E1265)

5789  [NCBI]

120&15 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.