2. Recombinant Proteins
  3. Viral Proteins Biotinylated Proteins
  4. SARS-CoV-2 Proteins
  5. SARS-CoV-2 3C-like Protease
  6. SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi)

SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi)

Cat. No.: HY-P76590
Handling Instructions

SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi) is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli , with N-His, N-Avi labeled tag. The total length of SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi) is 306 a.a., with molecular weight of ~36.5 kDa.

For research use only. We do not sell to patients.

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Description

SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi) is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli , with N-His, N-Avi labeled tag. The total length of SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi) is 306 a.a., with molecular weight of ~36.5 kDa.
Species

Virus
Source

E. coli
Tag

N-His;N-Avi
Accession

P0DTC1-1/YP_009725295.1 (S3264-Q3569)
Gene ID

43740578  [NCBI]
Molecular Construction
N-term
His-Avi
SARS-CoV-2 3CLpro (S3264-Q3569)
Accession # P0DTC1-1/YP_009725295.1
C-term
Synonyms
3CL Protease; 3CL Proteinase; 3C-like main protease; 3CL-Mpro; COVID-19; M Proteinase; Main Protease; Mpro
Molecular Weight

Approximately 36.5 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SARS-CoV-2 3CLpro/3C-like protease Protein (His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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