Semaphorin-4D/SEMA4D Protein, Cynomolgus (HEK293, His)

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling. It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner. Semaphorin-4D/SEMA4D Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Cynomolgus (HEK293, His) is 711 a.a., with molecular weight of 90-120 kDa.

Semaphorin-4D (SEMA4D) operates as a cell surface receptor for PLXNB1 and PLXNB2, assuming a crucial role in cell-cell signaling. This versatile protein is implicated in diverse cellular processes, including the regulation of GABAergic synapse development, where it fosters the development of inhibitory synapses in a PLXNB1-dependent manner. In hippocampal neurons, SEMA4D influences the complexity and arborization of developing neurites by activating PLXNB1, thereby mediating RHOA activation. Additionally, SEMA4D is involved in promoting the migration of cerebellar granule cells and induces B-cell aggregation, enhancing their viability in vitro. Furthermore, SEMA4D stimulates endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. Operating as a homodimer, SEMA4D interacts with both PLXNB1 and PLXNB2, underscoring its intricate involvement in various cellular and developmental processes.

Immobilized Cynomolgus Semaphorin 4D, His Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC₅₀ of ≤11.8 ng/mL determined by ELISA.

Cynomolgus

HEK293

C-His

A0A2K5TZC9 (F24-R734)

/

90-120 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.