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  4. Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, mFc)

Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, mFc)

Cat. No.: HY-P700429
Handling Instructions

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-mFc labeled tag.

For research use only. We do not sell to patients.

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Description

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-mFc labeled tag.
Background

Semaphorin-4D (SEMA4D) operates as a cell surface receptor for PLXNB1 and PLXNB2, assuming a crucial role in cell-cell signaling. This versatile protein is implicated in diverse cellular processes, including the regulation of GABAergic synapse development, where it fosters the development of inhibitory synapses in a PLXNB1-dependent manner. In hippocampal neurons, SEMA4D influences the complexity and arborization of developing neurites by activating PLXNB1, thereby mediating RHOA activation. Additionally, SEMA4D is involved in promoting the migration of cerebellar granule cells and induces B-cell aggregation, enhancing their viability in vitro. Furthermore, SEMA4D stimulates endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. Operating as a homodimer, SEMA4D interacts with both PLXNB1 and PLXNB2, underscoring its intricate involvement in various cellular and developmental processes.
Species

Mouse
Source

HEK293
Tag

C-mFc
Accession

O09126 (F24-R733)
Gene ID
Molecular Construction
N-term
SEMA4D (F24-R733)
Accession # O09126
mFc
C-term
Synonyms
Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
AA Sequence

FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR

Molecular Weight

108.1 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, mFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
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