Serpin A3 Protein, Human (HEK293, His)

Serpin A3 protein is a serine protease inhibitor on chromosome 14 that affects protease targeting through tissue-specific polymorphisms. Sequence variants are associated with Alzheimer's disease, while deficiencies are associated with liver disease. Serpin A3 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin A3 Protein, Human (HEK293, His) is 398 a.a., with molecular weight of ~50-70 kDa.

Serpin A3 Protein, a member of the serpin family, functions as an inhibitor of serine proteases. Situated on the q arm of chromosome 14, this gene is part of a cluster of serpin genes. Polymorphisms in Serpin A3 exhibit tissue-specific patterns and impact protease targeting. Variations in the protein's sequence have been implicated in Alzheimer's disease, and its deficiency is associated with liver disease. Notably, mutations in Serpin A3 have been identified in patients with Parkinson's disease and chronic obstructive pulmonary disease, emphasizing its potential role in diverse physiological conditions. The biased expression profile, with a prominent presence in the liver (RPKM 989.5), gall bladder (RPKM 89.2), and four other tissues, underscores its importance in various biological contexts.

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 is 14.968 nM, as measured under the described conditions.

Human

HEK293

C-His

NP_001076.2 (N26-A423)

12  [NCBI]

NSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

Approximately 50-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 25 mM HEPES, 0.15M NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.