Serpin A8/Angiotensinogen Protein, Human (HEK293, His, solution)

Serpin A8/angiotensinogen protein is an important component of the renin-angiotensin system (RAS), which regulates blood pressure, fluid balance, and electrolyte homeostasis. Serpin A8/Angiotensinogen Protein, Human (HEK293, His, solution) is the recombinant human-derived Serpin A8/Angiotensinogen protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin A8/Angiotensinogen Protein, Human (HEK293, His, solution) is 452 a.a., with molecular weight of 55.0-72.27 kDa.

Serpin A8, also known as angiotensinogen, stands as an indispensable element within the renin-angiotensin system (RAS), a potent regulatory framework governing blood pressure, body fluid, and electrolyte homeostasis. Operating directly on vascular smooth muscle, it exerts a formidable vasoconstrictive influence and dynamically influences cardiac contractility and heart rate through interactions with the sympathetic nervous system. Additionally, angiotensinogen plays a pivotal role in shaping renal sodium and water absorption, eliciting its effects by stimulating zona glomerulosa cells in the adrenal cortex to synthesize and release aldosterone. This multifaceted functionality is mediated through the binding of angiotensinogen to angiotensin receptors AGTR1 and AGTR2, orchestrating a cascade of physiological responses. Beyond its pivotal role in the RAS, angiotensinogen exhibits an ability to engage with the DEAR/FBXW7-AS1 receptor, expanding its repertoire of molecular interactions and suggesting broader implications for its regulatory influence.

Measured in a cell proliferation assay using HT-29 cells. The ED₅₀ for this effect is 54.57 ng/mL, corresponding to a specific activity is 1.833×10⁴ Unit/mg.

Human

HEK293

C-6*His

P01019/AAH11519.1 (D34-A485)

183  [NCBI]

DRVYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLMGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVPFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

55.0-72.27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.