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  5. Siglec-10 Protein, Cynomolgus (HEK293, His, solution)

Siglec-10 Protein, Cynomolgus (HEK293, His, solution)

Cat. No.: HY-P701083
Handling Instructions

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Cynomolgus (HEK293, His, solution) is the recombinant cynomolgus-derived Siglec-10 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-10 Protein, Cynomolgus (HEK293, His, solution) is 536 a.a., with molecular weight of 70-80 kDa.

For research use only. We do not sell to patients.

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Description

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Cynomolgus (HEK293, His, solution) is the recombinant cynomolgus-derived Siglec-10 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-10 Protein, Cynomolgus (HEK293, His, solution) is 536 a.a., with molecular weight of 70-80 kDa.

Background

Siglec-10 protein, recognized as a putative adhesion molecule, functions in sialic-acid dependent cellular binding, displaying a preference for alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site of Siglec-10 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it appears to act as an inhibitory receptor, inducing ligand-induced tyrosine phosphorylation and recruiting cytoplasmic phosphatases via their SH2 domains, blocking signal transduction through dephosphorylation of signaling molecules. Siglec-10 is involved in the negative regulation of B-cell antigen receptor signaling, dependent on PTPN6/SHP-1. In association with CD24, it may participate in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Siglec-10, in collaboration with CD24, regulates the immune response of natural killer (NK) cells and plays a role in the control of autoimmunity. During the initiation of adaptive immune responses by CD8-alpha(+) dendritic cells, Siglec-10 inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes, implicating the recruitment of PTPN6/SHP-1 and promoting phagosomal acidification. Siglec-10 interacts with various proteins, including PTPN6/SHP-1, NCF1, CD24, HMGB1, RIGI, CBL, and PTPN11.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5WBX8 (T17-N552)

Gene ID

102125591

Molecular Construction
N-term
Siglec-10 (T17-N552)
Accession # A0A2K5WBX8
His
C-term
Synonyms
SLG2; SIGLEC10; MGC126774; PRO940
Molecular Weight

70-80 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Siglec-10 Protein, Cynomolgus (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-10 Protein, Cynomolgus (HEK293, His, solution)
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HY-P701083
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