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  4. Siglec-2/CD22
  5. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His)

Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His)

Cat. No.: HY-P701288
Handling Instructions

Siglec-2/CD22, a B-cell surface receptor, mediates B-cell interactions and participates in B-cell localization within lymphoid tissues. It selectively binds alpha-2,6-linked sialic acid, including CD45, with its sialic acid recognition site potentially regulated by cis interactions. In the immune response, ligand-induced tyrosine phosphorylation suggests a role in B-cell antigen receptor signaling modulation. It engages in both positive and inhibitory regulation by interacting with Src family kinases and recruiting cytoplasmic phosphatases via their SH2 domains. Interactions include LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D, and GRB2 upon phosphorylation, as well as PTPN6/SHP-1 upon phosphorylation. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with His labeled tag. The total length of Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is 681 a.a., with molecular weight of 90-110 kDa.

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Description

Siglec-2/CD22, a B-cell surface receptor, mediates B-cell interactions and participates in B-cell localization within lymphoid tissues. It selectively binds alpha-2,6-linked sialic acid, including CD45, with its sialic acid recognition site potentially regulated by cis interactions. In the immune response, ligand-induced tyrosine phosphorylation suggests a role in B-cell antigen receptor signaling modulation. It engages in both positive and inhibitory regulation by interacting with Src family kinases and recruiting cytoplasmic phosphatases via their SH2 domains. Interactions include LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D, and GRB2 upon phosphorylation, as well as PTPN6/SHP-1 upon phosphorylation. Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with His labeled tag. The total length of Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His) is 681 a.a., with molecular weight of 90-110 kDa.

Background

Siglec-2/CD22 protein functions as a mediator of B-cell interactions and is potentially involved in the localization of B-cells within lymphoid tissues. This protein selectively binds to sialylated glycoproteins, with a preference for alpha-2,6-linked sialic acid, and one of its binding partners is CD45. The recognition site for sialic acid can be masked through cis interactions with sialic acids on the same cell surface. Upon ligand-induced tyrosine phosphorylation during immune responses, Siglec-2/CD22 is implicated in the regulation of B-cell antigen receptor signaling. It plays a dual role in signaling, positively regulating interactions with Src family tyrosine kinases and potentially acting as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains. This recruitment blocks signal transduction through the dephosphorylation of signaling molecules. In molecular interactions, Siglec-2/CD22 associates with proteins such as LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D, and GRB2 upon phosphorylation, suggesting its involvement in complex signaling networks that modulate B-cell responses.

Species

Mouse

Source

HEK293

Tag

His

Accession

P35329-1 (S22-P702)

Gene ID

12483

Synonyms
CD22; SIGLEC2; BL-CAM; SIGLEC-2; Siglec2; SIGLEC2FLJ22814
Molecular Weight

90-110 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-2/CD22 Protein, Mouse (Biotinylated, HEK293, His)
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HY-P701288
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