SPARCL1 Protein, Mouse (283a.a, HEK293, His)

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (283a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with N-His labeled tag.

SPARCL1 protein is a multifunctional molecule with calcium ion, collagen and extracellular matrix binding activities. It is mainly localized at glutamatergic synapses and participates in the synaptic membrane adhesion process. SPARCL1 is widely expressed in multiple systems, including cardiovascular, central nervous, male reproductive, respiratory and sensory organ systems, and is particularly highly expressed in adult bladder and cortex, suggesting that it plays a key role in these tissues. The protein is highly conserved in evolution, and the existence of its human homolog SPARC like 1 further confirms its functional importance.

Mouse

HEK293

N-6*His

NP_034227.3 (Y368-F650)

13602  [NCBI]

YLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Approximately 38-48 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.