SPG3A/ATL1 Protein, Human (sf9, His)

The SPG3A/ATL1 protein is a GTPase that promotes membrane tethering by forming trans homomers, thereby achieving homotypic fusion of the endoplasmic reticulum (ER) membrane. It makes a crucial contribution to the biogenesis of the endoplasmic reticulum tubular network and shaping the structural organization of cells. SPG3A/ATL1 Protein, Human (sf9, His) is the recombinant human-derived SPG3A/ATL1 protein, expressed by Sf9 insect cells , with N-His labeled tag.

SPG3A/ATL1 Protein functions as a GTPase that plays a pivotal role in membrane tethering through the formation of trans-homooligomers, facilitating homotypic fusion of endoplasmic reticulum (ER) membranes. Its involvement extends to endoplasmic reticulum tubular network biogenesis, contributing to the intricate organization of this cellular structure. Beyond its primary function, SPG3A/ATL1 may also participate in the regulation of Golgi biogenesis and axonal development. In its monomeric state as an apoprotein and in the GDP-bound form, SPG3A/ATL1 transforms into a homodimer when bound to GTP, demonstrating a dynamic interplay between its different states. The protein engages in various interactions with key cellular components, including MAP4K4, REEP5, RTN3, RTN4, SPAST, TMED2, REEP1, CPT1C, and ARL6IP1, highlighting its involvement in a complex network of molecular associations. Additionally, SPG3A/ATL1 interacts with ZFYVE27, further expanding its regulatory repertoire.

Human

Sf9 insect cells

N-10*His

Q8WXF7-1 (M1-T447)

51062  [NCBI]

MAKNRRDRNSWGGFSEKTYEWSSEEEEPVKKAGPVQVLIVKDDHSFELDETALNRILLSEAVRDKEVVAVSVAGAFRKGKSFLMDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWSEIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQEDDLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVSGNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILIPWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAAVATAKDTYNKKMEEICGGDKPFLAPNDLQTKHLQLKEESVKLFRGVKKMGGEEFSRRYLQQLESEIDELYIQYIKHNDSKNIFHAAR

Approximately 54 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

For concentration less than that, please reconstitute in 50 mM Tris-HCL, 500 mM NaCl, pH 8.0 buffer, 10% Glycerol.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.