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  5. TAOK3 Protein, Human (sf9, His)

TAOK3 Protein, Human (sf9, His)

Cat. No.: HY-P77222
SDS COA Handling Instructions

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TAOK3 Protein, Human (sf9, His) is 411 a.a..

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Description

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TAOK3 Protein, Human (sf9, His) is 411 a.a..

Background

TAOK3, a serine/threonine-protein kinase, functions as a regulator of the p38/MAPK14 stress-activated MAPK cascade and the MAPK8/JNK cascade. It acts as an activator of the p38/MAPK14 cascade, particularly in response to DNA damage, where it plays a role in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 cascade. This activation is likely mediated through the phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Moreover, TAOK3 serves as an inhibitor of the basal activity of the MAPK8/JNK cascade and reduces its activation in response to epidermal growth factor (EGF). These regulatory functions highlight TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling.

Biological Activity

Measured by its ability to catalyze 250 μg/mL PKCtide peptide substrate adenosine and 25 μM ATP that incubate at room temperature for 40 minutes. The specific activity is 32.49 nmo/min/mg.

Species

Human

Source

Sf9 insect cells

Tag

N-10*His

Accession

Q9H2K8 (M1-D411)

Gene ID
Molecular Construction
N-term
10*His
TAOK3 (M1-D411)
Accession # Q9H2K8
C-term
Synonyms
Serine/threonine-protein kinase TAO3; JNK/SAPK-inhibitory kinase; DPK; JIK; KDS; MAP3K18
AA Sequence

MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDRPLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNREMDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKDHVFIRDEAGHGD

Molecular Weight

Approximately 54 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 8.0, 10% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

TAOK3 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TAOK3 Protein, Human (sf9, His)
Cat. No.:
HY-P77222
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