TIM-3/HAVCR2 Protein, Human (Biotinylated, HEK293, His-Avi)

The TIM-3/HAVCR2 protein regulates immune responses to pathogens, interacting with LGALS9 on infected macrophages to limit bacterial growth. It enhances CD8+ T cell responses and acts as a phosphatidylserine receptor, mediating phagocytosis of apoptotic cells. TIM-3/HAVCR2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TIM-3/HAVCR2 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The protein being described is involved in the innate immune response to intracellular pathogens. It interacts with LGALS9 on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity and restrict bacterial growth. However, the role of this protein as a receptor for LGALS9 has been challenged. It also enhances CD8+ T-cell responses to acute infections and acts as a receptor for phosphatidylserine (PtSer), which is calcium-dependent. It may recognize PtSer on apoptotic cells and mediate their phagocytosis. The protein is expressed on T-cells and dendritic cells and promotes conjugation of apoptotic cells but not their engulfment. It positively regulates the innate immune response in dendritic cells and synergizes with Toll-like receptors to promote TNF-alpha secretion. In tumor-infiltrating dendritic cells, it suppresses nucleic acid-mediated innate immune response by interacting with HMGB1. It acts as a coreceptor on natural killer cells to enhance IFN-gamma production in response to LGALS9, but also suppresses NK cell-mediated cytotoxicity. It negatively regulates NK cell function in LPS-induced endotoxic shock. The protein interacts with various other proteins, including HMGB1, BAG6, PIK3R1, PIK3R2, FYN, VAV1, AKT1/2, LCP2, ZAP70, SYK, SH3BP2, SH2D2A, LCK, PLCG, and ILF3. Its interaction with ILF3 promotes ILF3 ubiquitination and degradation.

Immobilized Anti-Tim-3 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC₅₀ of 15.8 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q8TDQ0-1 (S22-R200)

84868  [NCBI]

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

45-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.