TPBG/5T4 Protein, Cynomolgus (HEK293, His)

TPBG/5T4 protein blocks Wnt3a-dependent LRP6 internalization by indirectly interacting with LRP6, thereby potentially inhibiting Wnt/β-catenin signaling. This unique mechanism suggests that TPBG/5T4 plays a crucial role in regulating the Wnt/β-catenin pathway by disrupting LRP6 internalization and affecting downstream signaling. TPBG/5T4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TPBG/5T4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TPBG/5T4 Protein, Cynomolgus (HEK293, His) is 321 a.a., with molecular weight of 55-80 kDa.

The TPBG/5T4 Protein emerges as a potential inhibitor of Wnt/beta-catenin signaling, operating through an indirect interaction with LRP6 and the blocking of Wnt3a-dependent LRP6 internalization. This distinctive mechanism suggests that TPBG/5T4 plays a crucial role in regulating the Wnt/beta-catenin pathway by disrupting the internalization process of LRP6, thereby influencing downstream signaling events. This insight into TPBG/5T4's function underscores its significance in modulating the intricate molecular dynamics associated with Wnt/beta-catenin signaling, offering a potential avenue for therapeutic exploration in the context of cellular regulatory processes.

Cynomolgus

HEK293

C-6*His

Q4R8Y9 (T35-S355)

102132149  [NCBI]

TSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS

55-80 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.