TRIM21 Protein, Human (P.pastoris, His)

TRIM21 Protein, an E3 ubiquitin ligase, plays a vital role in the immune response by targeting intracellular pathogens for degradation. It is involved in the recognition and clearance of viral particles. The therapeutic potential of targeting TRIM21 Protein in infectious diseases and its role in host defense make it a subject of interest in biomedical research. TRIM21 Protein, Human (P.pastoris, His) is the recombinant human-derived TRIM21 protein, expressed by P. pastoris , with N-His labeled tag. The total length of TRIM21 Protein, Human (P.pastoris, His) is 475 a.a., with molecular weight of ~56.2 kDa.

The protein being described is involved in various cellular processes and pathways. It is linked to the VCP-mediated ERAD pathway, which is responsible for eliminating misfolded proteins in the endoplasmic reticulum. It promotes the ubiquitination of IRF8, enhancing its ability to stimulate the transcription of cytokine genes in macrophages. It also plays a role in regulating cell cycle progression.

Human

P. pastoris

N-His

P19474 (1M-475Y)

6737  [NCBI]

MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

Approximately 56.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.