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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. TTK Protein, Human (Sf9, FLAG)

TTK Protein, Human (Sf9, FLAG)

Cat. No.: HY-P701654
Handling Instructions

The TTK protein is a highly efficient kinase that phosphorylates proteins on serine, threonine, and tyrosine residues, potentially regulating fundamental cellular processes. Notably, it phosphorylates MAD1L1, promoting mitotic checkpoint signaling, which is critical for accurate chromosome segregation. TTK Protein, Human (Sf9, FLAG) is the recombinant human-derived TTK protein, expressed by Sf9 insect cells , with C-Flag labeled tag and K857G, , , , mutation. The total length of TTK Protein, Human (Sf9, FLAG) is 857 a.a., .

For research use only. We do not sell to patients.

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Description

The TTK protein is a highly efficient kinase that phosphorylates proteins on serine, threonine, and tyrosine residues, potentially regulating fundamental cellular processes. Notably, it phosphorylates MAD1L1, promoting mitotic checkpoint signaling, which is critical for accurate chromosome segregation. TTK Protein, Human (Sf9, FLAG) is the recombinant human-derived TTK protein, expressed by Sf9 insect cells , with C-Flag labeled tag and K857G, , , , mutation. The total length of TTK Protein, Human (Sf9, FLAG) is 857 a.a., .

Background

TTK, a proficient kinase, exhibits versatile substrate specificity by phosphorylating proteins on serine, threonine, and tyrosine residues. Implicating its role in cell proliferation, TTK likely plays a crucial part in regulating fundamental cellular processes. Notably, TTK phosphorylates MAD1L1, thereby promoting mitotic checkpoint signaling, a pivotal mechanism for ensuring accurate chromosome segregation during cell division. Additionally, TTK's significance extends to chromosome alignment, where it enhances the activity of AURKB by directly phosphorylating CDCA8 at the centromere. The intricate involvement of TTK in mitotic processes underscores its essential role in safeguarding genomic stability and orchestrating key events during cell division.

Species

Human

Source

Sf9 insect cells

Tag

C-Flag

Accession

P33981 (M1-K857, K857G)

Gene ID

7272

Molecular Construction
N-term
TTK (M1-K857, K857G)
Accession # P33981
Flag
C-term
Synonyms
TTK; Dual specificity protein kinase TTK; Phosphotyrosine picked threonine-protein kinase; PYT
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

TTK Protein, Human (Sf9, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TTK Protein, Human (Sf9, FLAG)
Cat. No.:
HY-P701654
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