USP3 Protein, Human (Active, Sf9, GST)

USP3 Protein, Human (Active, Sf9, GST) is the recombinant human-derived USP3, expressed by Sf9 insect cells , with GST labeled tag. ,

The fundamental role of USP3 is specific removal of ubiquitin from substrates. USP3 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP3 used is 1.5 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.

Human

Sf9 insect cells

N-GST

Q9Y6I4-1 (E2-L520)

9960

Approximately 85.4 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.