USP46 Protein, Human

USP46 protein, as a deubiquitinating enzyme, may participate in behavioral regulation by regulating the effects of GABA. Suggested to mediate deubiquitination of GAD1/GAD67, affecting processes related to GABAergic neurotransmission. USP46 Protein, Human is the recombinant human-derived USP46 protein, expressed by E. coli , with tag free. The total length of USP46 Protein, Human is 366 a.a., .

USP46 is identified as a behaviorally relevant deubiquitinating enzyme, potentially influencing behavior through the regulation of GABAergic neurotransmission. Its role is suggested to involve mediating the deubiquitination of GAD1/GAD67, crucial in GABA synthesis. Remarkably, USP46 exhibits minimal deubiquitinating activity independently, necessitating interaction with WDR48 for substantial enzymatic function. This intricate partnership with WDR48 highlights the collaborative nature of its enzymatic activity. Notably, USP46 does not participate in the deubiquitination of monoubiquitinated FANCD2, indicating specificity in its cellular functions.

The fundamental role of USP46 is specific removal of ubiquitin from substrates. USP46 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM.

Human

E. coli

Tag Free

P62068 (M1-E366)

64854

MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Approximately 42.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 10% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.