USP47 Protein, Human (Sf9, FLAG)

The USP47 protein is a ubiquitin-specific protease that deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizes POLB, and regulates base excision repair (BER). In addition to DNA repair, USP47 is an important regulator of cell growth and genome integrity. USP47 Protein, Human (Sf9, FLAG) is the recombinant human-derived USP47 protein, expressed by Sf9 insect cells , with C-Flag labeled tag. The total length of USP47 Protein, Human (Sf9, FLAG) is 1374 a.a., .

USP47 protein functions as a ubiquitin-specific protease with a specific role in the deubiquitination of monoubiquitinated DNA polymerase beta (POLB), thereby stabilizing POLB and contributing to the regulation of base-excision repair (BER). Beyond its role in DNA repair, USP47 emerges as a key regulator of cell growth and genome integrity. Additionally, it may exert its influence on cellular processes by indirectly modulating CDC25A expression at the transcriptional level, underscoring its multifaceted role in cellular homeostasis and genomic stability.

The fundamental role of USP47 is specific removal of ubiquitin from substrates. USP47 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission ligat under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

C-Flag

Q96K76 (V2-D1375)

55031

Approximately 158.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, pH 7.5, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.