USP48 Protein, Human (Sf9, FLAG)

USP48 protein, a deubiquitinase, hydrolyzes ubiquitin bonds at the C-terminal Gly, processing poly-ubiquitin precursors and ubiquitinated proteins. It may regulate NF-kappa-B activation by the TNF receptor superfamily via RELA and TRAF2 interactions. With potential roles at postsynaptic sites, USP48 exhibits multifunctionality, influencing ubiquitin processing and signaling pathways involved in immune response and synaptic function. USP48 Protein, Human (Sf9, FLAG) is the recombinant human-derived USP48 protein, expressed by Sf9 insect cells , with C-Flag labeled tag. The total length of USP48 Protein, Human (Sf9, FLAG) is 1034 a.a., .

The USP48 protein functions as a deubiquitinase, specifically recognizing and hydrolyzing the peptide bond at the C-terminal Gly of ubiquitin. It is implicated in the processing of both poly-ubiquitin precursors and ubiquitinated proteins. Additionally, USP48 may play a role in regulating NF-kappa-B activation by the TNF receptor superfamily through interactions with RELA and TRAF2. Furthermore, it is suggested to have a potential regulatory function at postsynaptic sites. These diverse activities highlight the multifunctional nature of USP48 in cellular processes, encompassing ubiquitin processing and modulation of signaling pathways implicated in immune response and synaptic function.

The fundamental role of USP48 is specific removal of ubiquitin from substrates. USP48 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

C-Flag

Q86UV5 (A2-H1035)

84196

Approximately 120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.