VCAM-1/CD106 Protein, Human (HEK293, Fc)

VCAM-1/CD106 proteins expressed on endothelial cells critically regulate leukocyte adhesion through integrin interactions, initiating calcium channel activation and RAC1-mediated transendothelial migration during inflammation. VCAM-1/CD106 Protein, Human (HEK293, Fc) is the recombinant human-derived VCAM-1/CD106 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of VCAM-1/CD106 Protein, Human (HEK293, Fc) is 673 a.a., with molecular weight of 110-150 kDa.

VCAM-1/CD106 is a cell adhesion glycoprotein predominantly expressed on endothelial cells, playing a pivotal role in immune surveillance and inflammation. As a major regulator of leukocyte adhesion to the endothelium, it interacts with various integrins, influencing immune cell interactions. During inflammatory responses, VCAM-1 binds ligands on activated endothelial cells, initiating calcium channel activation and RAC1 small GTPase activation, facilitating leukocyte transendothelial migration. Additionally, VCAM-1 acts as a quality-control checkpoint for entry into the bone marrow, providing a 'don't-eat-me' signal in the context of major histocompatibility complex (MHC) class-I presentation.

Measured by the ability of the proliferation protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ this effect is 0.1999 ng/ml, corresponding to a specific activity is 5.003×10⁶ units/mg.

• 
  Measured by the ability of the proliferation protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ this effect is 0.1999 ng/mL, corresponding to a specific activity is 5.003×10⁶ units/mg.

Human

HEK293

C-hFc

P19320 (F25-P697)

7412  [NCBI]

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSP

110-150 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.