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Vitamin D Receptor/VDR Protein, Mouse (sf9, His)

Cat. No.: HY-P73542
Handling Instructions

Vitamin D receptor (VDR) protein is the nuclear receptor of calcitriol, which translocates to the nucleus after binding vitamin D3, thereby coordinating cellular activities.VDR forms a heterodimer with RXR, binds to DNA response elements, and initiates the transcription of vitamin D3-responsive genes.Vitamin D Receptor/VDR Protein, Mouse (sf9, His) is the recombinant mouse-derived Vitamin D Receptor/VDR protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

Vitamin D receptor (VDR) protein is the nuclear receptor of calcitriol, which translocates to the nucleus after binding vitamin D3, thereby coordinating cellular activities.VDR forms a heterodimer with RXR, binds to DNA response elements, and initiates the transcription of vitamin D3-responsive genes.Vitamin D Receptor/VDR Protein, Mouse (sf9, His) is the recombinant mouse-derived Vitamin D Receptor/VDR protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

Vitamin D Receptor (VDR) Protein serves as a nuclear receptor for calcitriol, the active form of vitamin D3, mediating its cellular actions. Upon binding vitamin D3, VDR translocates into the nucleus, forming heterodimers with the retinoid X receptor (RXR). The VDR-RXR heterodimers then bind to specific response elements on DNA, initiating the transcription of vitamin D3-responsive target genes. Beyond its central role in calcium homeostasis, VDR also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites. In the absence of bound vitamin D3, VDR forms homodimers, while vitamin D3 binding prompts heterodimerization with RXRA. VDR interacts with various coactivators, including MED1, NCOA1, NCOA2, NCOA3, and NCOA6, leading to enhanced transcription of target genes. It also interacts with the corepressor NCOR1, SNW1, IRX4, and exhibits ligand-dependent interactions with CRY1 and CRY2. These molecular interactions highlight the complex regulatory network that governs the transcriptional activity of VDR in response to vitamin D3 and other ligands.

Species

Mouse

Source

Sf9 insect cells

Tag

C-His

Accession

P48281 (M1-S422)

Gene ID
Molecular Construction
N-term
VDR (M1-S422)
Accession # P48281
His
C-term
Synonyms
Vitamin D3 receptor; VDR; NR1I1
Molecular Weight

Approximately 55 kDa

Purity

Greater than 86% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Vitamin D Receptor/VDR Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vitamin D Receptor/VDR Protein, Mouse (sf9, His)
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HY-P73542
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