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  3. Von Willebrand Factor/vWF Protein, Human (CHO, His)

Von Willebrand Factor/vWF Protein, Human (CHO, His)

Cat. No.: HY-P73541
SDS COA Handling Instructions

Von Willebrand Factor/vWF Protein, Human (CHO, His) is the recombinant human-derived Von Willebrand Factor/vWF protein, expressed by CHO , with C-His labeled tag. The total length of Von Willebrand Factor/vWF Protein, Human (CHO, His) is 2791 a.a., with molecular weight of ~260 & 350 kDa, respectively.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
50 μg $378 In-stock
100 μg $643 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

Von Willebrand Factor/vWF Protein, Human (CHO, His) is the recombinant human-derived Von Willebrand Factor/vWF protein, expressed by CHO , with C-His labeled tag. The total length of Von Willebrand Factor/vWF Protein, Human (CHO, His) is 2791 a.a., with molecular weight of ~260 & 350 kDa, respectively.

Biological Activity

Immobilized BDD-FVIII at 2 μg/mL (100 μL/well) can bind Recombinant Human vWF Protein (His Tag), with a linear range of 0.16-4.0 μg/mL

Species

Human

Source

CHO

Tag

C-His

Accession

AAB59458.1 (A23-K2813)

Gene ID
Molecular Construction
N-term
vWF (A23-K2813)
Accession # AAB59458.1
His
C-term
Synonyms
von Willebrand factor; vWF; von Willebrand antigen II; F8VWF
Molecular Weight

Approximately 260&350 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Von Willebrand Factor/vWF Protein, Human (CHO, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Von Willebrand Factor/vWF Protein, Human (CHO, His)
Cat. No.:
HY-P73541
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