YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO)

YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag. The total length of YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is 181 a.a., with molecular weight of ~35.3 kDa.

YUAB, a key player in biofilm formation, engages in self-polymerization, creating a distinctive layer on the biofilm surface that imparts hydrophobic properties. This layer demonstrates stability and resilience, displaying resistance to significant mechanical force compression. Crucial for the development of complex colony architecture, YUAB is implicated in synergistic interactions with exopolysaccharides and TasA amyloid fibers, facilitating the assembly of the biofilm matrix. As a polymer-forming entity, YUAB contributes to the structural integrity and hydrophobic characteristics of the biofilm.

Others

E. coli Cell-free

N-His;N-SUMO

P71014 (M1-N181)

938831  [NCBI]

MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN

Approximately 35.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.