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ZFAND5 Protein, Human (Sf9, His, Strep)

Cat. No.: HY-P701604
Handling Instructions

The ZFAND5 protein is a key player in protein degradation by the ubiquitin-proteasome system and serves as a potential anchor for ubiquitinated proteins, promoting their proteasomal targeting. It regulates NF-kappa-B activation and apoptosis by inhibiting NF-kappa-B activation induced by various stimuli, including RIPK1 and TRAF6 overexpression. ZFAND5 Protein, Human (Sf9, His, Strep) is the recombinant human-derived ZFAND5 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of ZFAND5 Protein, Human (Sf9, His, Strep) is 212 a.a., .

For research use only. We do not sell to patients.

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Description

The ZFAND5 protein is a key player in protein degradation by the ubiquitin-proteasome system and serves as a potential anchor for ubiquitinated proteins, promoting their proteasomal targeting. It regulates NF-kappa-B activation and apoptosis by inhibiting NF-kappa-B activation induced by various stimuli, including RIPK1 and TRAF6 overexpression. ZFAND5 Protein, Human (Sf9, His, Strep) is the recombinant human-derived ZFAND5 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of ZFAND5 Protein, Human (Sf9, His, Strep) is 212 a.a., .

Background

ZFAND5 protein participates in the intricate processes of protein degradation via the ubiquitin-proteasome system, potentially serving as an anchor for ubiquitinated proteins to facilitate their targeting to the proteasome. Its involvement extends to the regulation of NF-kappa-B activation and apoptosis, exerting inhibitory effects on NF-kappa-B activation induced by various stimuli, including overexpression of RIPK1 and TRAF6, while not affecting RELA-triggered activation. ZFAND5 demonstrates a dose-dependent inhibition of NF-kappa-B activation in response to TNF, IL-1, and TLR4 stimuli and sensitizes cells to TNF-induced apoptosis. Notably, it acts as a potent inhibitory factor in osteoclast differentiation. Functionally, ZFAND5 interacts with ubiquitin and polyubiquitinated proteins and forms a heterotrimeric complex with ubiquitin and SQSTM1, with both ZFAND5 and SQSTM1 binding to the same ubiquitin molecule, emphasizing its role in coordinating ubiquitin-mediated processes. Additionally, ZFAND5 engages in interactions with IKBKG, RIPK1, and TRAF6 through specific domains, further underscoring its involvement in crucial cellular pathways.

Species

Human

Source

Sf9 insect cells

Tag

N-Strep;N-8*His

Accession

O76080 (A2-I213)

Gene ID

7763

Molecular Construction
N-term
8*His-Strep
ZFAND5 (A2-I213)
Accession # O76080
C-term
Synonyms
ZFAND5; AN1-type zinc finger protein 5; Zinc finger A20 domain-containing protein 2; Zinc finger protein 216
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ZFAND5 Protein, Human (Sf9, His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ZFAND5 Protein, Human (Sf9, His, Strep)
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HY-P701604
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