ZFAND5 Protein, Human (Sf9)

The ZFAND5 protein is a key player in protein degradation by the ubiquitin-proteasome system and serves as a potential anchor for ubiquitinated proteins, promoting their proteasomal targeting. It regulates NF-kappa-B activation and apoptosis by inhibiting NF-kappa-B activation induced by various stimuli, including RIPK1 and TRAF6 overexpression. ZFAND5 Protein, Human (Sf9) is the recombinant human-derived ZFAND5 protein, expressed by Sf9 insect cells , with tag free. The total length of ZFAND5 Protein, Human (Sf9) is 212 a.a., .

ZFAND5 protein participates in the intricate processes of protein degradation via the ubiquitin-proteasome system, potentially serving as an anchor for ubiquitinated proteins to facilitate their targeting to the proteasome. Its involvement extends to the regulation of NF-kappa-B activation and apoptosis, exerting inhibitory effects on NF-kappa-B activation induced by various stimuli, including overexpression of RIPK1 and TRAF6, while not affecting RELA-triggered activation. ZFAND5 demonstrates a dose-dependent inhibition of NF-kappa-B activation in response to TNF, IL-1, and TLR4 stimuli and sensitizes cells to TNF-induced apoptosis. Notably, it acts as a potent inhibitory factor in osteoclast differentiation. Functionally, ZFAND5 interacts with ubiquitin and polyubiquitinated proteins and forms a heterotrimeric complex with ubiquitin and SQSTM1, with both ZFAND5 and SQSTM1 binding to the same ubiquitin molecule, emphasizing its role in coordinating ubiquitin-mediated processes. Additionally, ZFAND5 engages in interactions with IKBKG, RIPK1, and TRAF6 through specific domains, further underscoring its involvement in crucial cellular pathways.

Human

Sf9 insect cells

Tag Free

O76080 (A2-I213)

7763

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.