2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Tetrahydrofolic acid trihydrochloride

Tetrahydrofolic acid trihydrochloride  (Synonyms: L-5,6,7,8-Tetrahydrofolic acid trihydrochloride; L-Tetrahydrofolic acid trihydrochloride)

Cat. No.: HY-14520B
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Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

For research use only. We do not sell to patients.

Tetrahydrofolic acid trihydrochloride Chemical Structure

Tetrahydrofolic acid trihydrochloride Chemical Structure

CAS No. : 150731-85-4

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Other Forms of Tetrahydrofolic acid trihydrochloride:

Tetrahydrofolic acid trihydrochloride Related Antibodies

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Description

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) trihydrochloride is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid trihydrochloride is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid trihydrochloride serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Tetrahydrofolic acid (0-200 μM; 3 days; Adh5-/- DT40 cells) trihydrochloride exposure is cytotoxic to Adh5- and Fanconi anemia (FA)-deficient cells due to the accumulation of extensive DNA damage and chromosome breaks[1].
Tetrahydrofolic acid (0-100 μM; 16 hours; Adh5-/- DT40 cells) trihydrochloride strongly promots FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner[1].
Tetrahydrofolic acid trihydrochloride exposure activates the DNA damage response (DDR) due to uncontrolled activity of the thymidylate synthase enzyme, which causes a depletion of essential nucleotides, and promotes repair by a homologous recombination mechanism[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tetrahydrofolic acid trihydrochloride Related Antibodies

Cell Viability Assay[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 3 days
Result: Viability of Adh5-/-DT40 cells rapidly dropped.

Western Blot Analysis[1]

Cell Line: Adh5-/- DT40 cells
Concentration: 0-200 µM
Incubation Time: 16 hours
Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.
In Vivo

Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily; Adh5-/- mice) trihydrochloride perturbs the hematopoiesis of hematopoietic cells, increases ser139-H2AX phosphorylation, and decreases the survival of progenitor cells (HSPCs) suggesting that excess Tetrahydrofolic acid trihydrochloride could be mutagenic and genotoxic to bone marrow cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adh5-/- mice[1]
Dosage: 62.5 mg/kg
Administration: Intraperitoneal injection; daily
Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
Molecular Weight

554.81

Formula

C19H26Cl3N7O6
CAS No.
SMILES

NC(NC1=C2NC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)CN1)=NC2=O.Cl.Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Tetrahydrofolic acid trihydrochloride Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetrahydrofolic acid trihydrochloride150731-85-4L-5,6,7,8-Tetrahydrofolic acid trihydrochlorideL-Tetrahydrofolic acid trihydrochlorideEndogenous MetaboliteFolatederivativewater-solublevitamin5,10-methylenetetrahydrofolatehematopoiesisFANCD2one-carbonDNAaminonucleiclipidsInhibitorinhibitorinhibit

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