Trichostatin C

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Trichostatin C is an inhibitor for histone deacetylase (HDAC), induces apoptosis and arrests cell cycle at G2/M phase, and exhibits anticancer activity against lung cancer and urothelial bladder cancer. Trichostatin C induces differentation of Friend leukemic cells. Trichostatin C exhibits antifungal activity.

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Trichostatin C Chemical Structure

CAS No. : 68676-88-0

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•Related Proteins:

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View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
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Description

Trichostatin C is an inhibitor for histone deacetylase (HDAC), induces apoptosis and arrests cell cycle at G2/M phase, and exhibits anticancer activity against lung cancer and urothelial bladder cancer^[1]. Trichostatin C induces differentation of Friend leukemic cells^[2]. Trichostatin C exhibits antifungal activity^[3].

IC₅₀ & Target

HDAC1

HDAC6

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	> 100 μM Compound: 10	Cytotoxicity against human HEK293 cells after 96 hrs by MTT assay Cytotoxicity against human HEK293 cells after 96 hrs by MTT assay	[PMID: 25556102]
HeLa	IC₅₀	> 200 μM Compound: 10	Cytotoxicity against human HeLa cells after 96 hrs by MTT assay Cytotoxicity against human HeLa cells after 96 hrs by MTT assay	[PMID: 25556102]
HepG2	IC₅₀	> 200 μM Compound: 10	Cytotoxicity against human HepG2 cells after 96 hrs by MTT assay Cytotoxicity against human HepG2 cells after 96 hrs by MTT assay	[PMID: 25556102]
HepG2	EC₅₀	46.02 μM Compound: 10	Up-regulation of transcriptional activity of human CLA-1 promoter region -1055 to -62bp transfected in HepG2 cells by luciferase reporter gene assay Up-regulation of transcriptional activity of human CLA-1 promoter region -1055 to -62bp transfected in HepG2 cells by luciferase reporter gene assay	[PMID: 25556102]
L02	IC₅₀	> 200 μM Compound: 10	Cytotoxicity against human LO2 cells after 96 hrs by MTT assay Cytotoxicity against human LO2 cells after 96 hrs by MTT assay	[PMID: 25556102]
SW480	IC₅₀	72 μM Compound: 5	Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 72 hrs by EZ-Cytox colorimetric assay Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 72 hrs by EZ-Cytox colorimetric assay	[PMID: 34455777]
U2OS	IC₅₀	> 200 μM Compound: 10	Cytotoxicity against human U2OS cells after 96 hrs by MTT assay Cytotoxicity against human U2OS cells after 96 hrs by MTT assay	[PMID: 25556102]
U2OS	IC₅₀	94.9 μM Compound: 5	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by EZ-Cytox colorimetric assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by EZ-Cytox colorimetric assay	[PMID: 34455777]

In Vitro

Trichostatin C (0-100 μM) exhibits inhibitory activity against HDAC1 and HDAC6, inhibits proliferations of cancer cells A549, J82 and SK-BR-3, with IC₅₀s of 6.24, 4.16 and 0.6 μM, respectively^[1].
Trichostatin C (0-10 μM) induces apoptosis in cells J82 through activation of caspase 3 and caspase 7^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	A549, J82 and SK-BR-3
Concentration:	0-100 μM
Incubation Time:	72 h
Result:	Inhibits proliferations of A549, J82 and SK-BR-3.

Western Blot Analysis^[1]

Cell Line:	J82
Concentration:	20-80 μM
Incubation Time:	24 h
Result:	Induced acetylation of α-Tubulin and Histone 3 (markers of HDAC1 and HDAC6 inhibition).

Apoptosis Analysis^[1]

Cell Line:	J-82
Concentration:	0.1-10 μM
Incubation Time:	48 h
Result:	Activated 23.1, 61.7 and 62.3% caspase 3/7 at 0.1 μM, 1 μM and 10 μM.

Molecular Weight

464.51

Formula

C₂₃H₃₂N₂O₈

CAS No.

68676-88-0

SMILES

O=C(NO[C@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O)/C=C/C(C)=C/[C@@H](C)C(C2=CC=C(N(C)C)C=C2)=O

Structure Classification

Others

Initial Source

Microorganisms

Streptomyces sp. RK98-A74

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Wang C, et al., Trichostatin C Synergistically Interacts with DNMT Inhibitor to Induce Antineoplastic Effect via Inhibition of Axl in Bladder and Lung Cancer Cells[J]. Pharmaceuticals, 2024, 17(4): 425.

[2]. Yoshida M, et al., Trichostatin C, a new inducer of differentiation of Friend leukemic cells[J]. Agricultural and biological chemistry, 1985, 49(2): 563-565.

[3]. Tsuji N, et al., a glucopyranosyl hydroxamate. J Antibiot (Tokyo). 1978 Oct;31(10):939-44. [Content Brief]

Trichostatin C Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trichostatin C68676-88-0FungalHDACApoptosisHistone deacetylasesLeukemialung cancerurothelial bladder cancerbreast cancerA549J82SK-BR-3Inhibitorinhibitorinhibit

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