Tyrosinase, Mushroom  (Synonyms: Polyphenol oxidase)

Tyrosinase (EC 1.14.18.1) (Polyphenol oxidase) is a rate-limiting enzyme that controls the production of melanin and is encoded by TYR gene. Tyrosinase is mainly found in melanosomes synthesized by skin melanocytes^[1].

Product Information
Optimal pH: 6.0 - 7.0
Theoretical molecular weight: 128 kDa (a tetramer)

Tyrosinase activity assay
1. Preparation of enzyme solution
Dissolve in ddH2O at a concentration of 1 mg/mL. Further dilute to a concentration of 200-400 U/mL.
2. Experimental method
1) Adjust the spectrophotometer to 280 nm and 25°C. Add samples to each colorimetric tube in the following order:
1mL 0.5 M Phosphate buffer, pH 6.5
1 mL 0.001 M L-Tyrosine
0.9 mL ddH2O
2) Fill the colorimetric tube with oxygen through the capillary tube for 4-5 minutes to oxidize the reaction mixture. Place the colorimetric tube on the spectrophotometer and record the A280 at 4-5 minutes as the absorbance of the blank group. Add 0.1 mL of appropriately diluted enzyme and record the A280 at 10-12 minutes.
3. Calculate
1U = the amount of enzyme that causes an increase of 0.001 in absorbance at 280 nm per minute.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9002-10-2

Solid

Light brown to brown

[Tyrosinase, Mushroom]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sánchez-Ferrer A, et al. Tyrosinase: a comprehensive review of its mechanism. Biochim Biophys Acta. 1995 Feb 22;1247(1):1-11.  [Content Brief]