UCK2 Inhibitor-3 

UCK2 Inhibitor-3 is a non-competitive inhibitor of uridine-cytidine kinase 2 (UCK2, a pyrimidine salvage enzyme) with an IC₅₀ value of 16.6 μM. UCK2 can replace dihydroorotate dehydrogenase (DHODH), in a certain extent, in infected or rapidly dividing cells to continue efficient uridine salvage. UCK2 Inhibitor-3 also inhibits DNA polymerase eta and kappa with IC₅₀s of 56 μM and 16 μM^[1].

IC50: 16.6 μM (UCK2); 56 μM (DNA polymerase η); 16 μM (DNA polymerase κ)^[1]

Dihydroorotate dehydrogenase (DHODH), a rate-limiting enzyme in mammalian de novo pyrimidine synthesis. UCK2 can make up for DHODH in a certain extent, in infected or rapidly dividing cells for uridine salvage. DHODH inhibition has antiviral and anticancer significance, while UCK2 Inhibitor-3 suppress nucleoside salvage in cells both in the presence and absence of DHODH inhibitors^[1].
UCK2 Inhibitor-3 (compound 135416439) is an inhibitor against pyrimidine salvage enzyme, non-competitive with Uridine and ATP with K_i values of 13 μM and 12 μM, respectively^[1].
UCK2 Inhibitor-3 (50 μM) inhibits UCK2 with inhibition rate of 31.3%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

474.31

C₁₉H₁₃BrFN₅O₂S

2376687-49-7

O=C(NC1=CC=C(Br)C=C1)CSC(N=C2C3=CNN2C4=CC=C(F)C=C4)=NC3=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Okesli-Armlovich A, et al. Discovery of small molecule inhibitors of human uridine-cytidine kinase 2 by high-throughput screening. Bioorg Med Chem Lett. 2019 Sep 15;29(18):2559-2564.  [Content Brief]