ZIKV-IN-1

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ZIKV-IN-1 is a potent zika virus inhibitor with an EC₅₀ of 2.8 μM and EC₉₀ of 6.8 μM. ZIKV-IN-1 shows anti-ZIKV activity with low cytotoxicity. ZIKV-IN-1 shows a strong affinity to ZIKV RdRp domain. ZIKV-IN-1 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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ZIKV-IN-1 Chemical Structure

CAS No. : 2762166-06-1

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Biological Activity
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Description

ZIKV-IN-1 is a potent zika virus inhibitor with an EC₅₀ of 2.8 μM and EC₉₀ of 6.8 μM. ZIKV-IN-1 shows anti-ZIKV activity with low cytotoxicity. ZIKV-IN-1 shows a strong affinity to ZIKV RdRp domain^[1]. ZIKV-IN-1 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

EC₅₀: 2.8 µM (ZIKV)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	> 50 μM Compound: 38	Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 35306290]
Huh-7	CC₅₀	43.8 μM Compound: 38	Cytotoxicity against human Huh-7 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human Huh-7 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 35306290]
SNB-19	CC₅₀	49.4 μM Compound: 38	Cytotoxicity against human SNB-19 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human SNB-19 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 35306290]
Vero	CC₅₀	46.8 μM Compound: 38	Cytotoxicity against African green monkey Vero cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 35306290]

In Vitro

ZIKV-IN-1 (compound 38) (0-80 µM) shows anti-ZIKV activity in a dose-dependent manner in A549 cells^[1].
ZIKV-IN-1 (5, 10, 25 µM; 48 h) decreases the expression of ZIKV E and cleaved caspase 3 protein^[1].
ZIKV-IN-1 (1.25, 2.5, 5, 10, 20 µM; 0-250 s) shows strong affinity to ZIKV RdRp domain (K_d=1.87 µM) in A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	A549 cells
Concentration:	0-50 µM
Incubation Time:
Result:	Showed anti-ZIKV activity with an EC₅₀ of 2.8 µM, EC₉₀ of 6.8 µM, and CC₅₀ of >50 µM.

Western Blot Analysis^[1]

Cell Line:	A549, Vero cells
Concentration:	5, 10, 25 µM
Incubation Time:	48 h
Result:	Decreased the expression of ZIKV E and cleaved caspase 3 protein.

Cell Cytotoxicity Assay^[1]

Cell Line:	Vero, SNB19, Huh7, A549 cells
Concentration:	0-80 µM
Incubation Time:
Result:	Showed low cytotoxicity with CC₅₀s of 46.8, 49.4, 43.8, 54.1 µM for ZG01 strain of Vero, SNB19, Huh7, A549 cells, and CC₅₀s of 470.5, 485.2, 512.8, 160.8 µM for MR766 strain of Vero, SNB19, Huh7, A549 cells, respectively.

Molecular Weight

478.29

Formula

C₂₁H₁₈BrF₂N₃O₃

CAS No.

2762166-06-1

SMILES

CC1=NC=NC2=C1C(C#CC3=CC(F)=C(C=C3)Br)=CN2[C@@H]4O[C@@H]([C@H]([C@]4(F)C)O)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Yao G, et al. Design, synthesis, and biological evaluation of novel 2'-methyl-2'-fluoro-6-methyl-7-alkynyl-7-deazapurine nucleoside analogs as anti-Zika virus agents. Eur J Med Chem. 2022 Apr 15;234:114275. [Content Brief]

ZIKV-IN-1 Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZIKV-IN-12762166-06-1Virus ProteaseFlaviviruslow cytotoxicityA549 cellsanti-ZIKVcleaved caspase 3 ZIKV RdRp dominZIKV E proteinInhibitorinhibitorinhibit

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