α-2,6-Sialyltransferase, pasteurella multocida (P-1059) 

alpha-2,6-Sialyltransferase (Pd26ST) (EC 2.4.99.1) is capable of sialylating both terminal and internal galactose and N-acetylgalactosamine, which producing terminal α2,6-sialosides^[1].

Product Information
This product is recombinantly expressed in Escherichia coli, featuring a C-terminal His tag. It has a molecular weight of approximately 54 kDa and a purity greater than 90% (SDS-PAGE). The storage buffer consists of 20 mM Tris-HCl at pH 8.0 and 10% glycerol.

Instructions for Use
1. Add substrate according to the reaction system, 5mM MgCl₂, and appropriate buffer salt (such as Tris-HCl), adjust the pH to 8.5, and add appropriate amount of α-2,6-Sialyltransferase (according to the reaction requirements);
2. After mixing, incubate the sample at 37℃ for 2-4h.
Note: When the system is amplified, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and low-speed shaking can be used for mixing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9075-81-4

Liquid

Colorless to light yellow

[a-2,6-Sialyltransferase, pasteurella multocida (P-1059)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yu H, et, al. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural alpha-2,6-linked sialosides: a P. damsela alpha-2,6-sialyltransferase with extremely flexible donor-substrate specificity. Angew Chem Int Ed Engl. 2006 Jun 12;45(24):3938-44.  [Content Brief]