1. Anti-infection
  2. Influenza Virus
  3. 2'-Deoxy-2'-fluorocytidine

2'-Deoxy-2'-fluorocytidine, an nucleoside analog, is a potent inhibitor of Crimean-Congo hemorrhagic fever virus (CCHFV) replication. 2′-deoxy-2′-fluorocytidine can act synergistically with T705 to increase the potency of both compounds antiviral effects on CCHFV replication.

For research use only. We do not sell to patients.

2'-Deoxy-2'-fluorocytidine Chemical Structure

2'-Deoxy-2'-fluorocytidine Chemical Structure

CAS No. : 10212-20-1

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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 2'-Deoxy-2'-fluorocytidine

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2'-Deoxy-2'-fluorocytidine, an nucleoside analog, is a potent inhibitor of Crimean-Congo hemorrhagic fever virus (CCHFV) replication. 2′-deoxy-2′-fluorocytidine can act synergistically with T705 to increase the potency of both compounds antiviral effects on CCHFV replication[1].

Cellular Effect
Cell Line Type Value Description References
HEp-2 EC50
> 200 μM
Compound: 2'd2'FC
Antiviral activity against RSV A2 infected in human Hep2 cells assessed as protection against virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay
Antiviral activity against RSV A2 infected in human Hep2 cells assessed as protection against virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay
[PMID: 25978965]
HEp-2 IC50
1.8 μM
Compound: 2'd2'FC
Inhibition of RSV A2 RNA-dependent RNA polymerase expressed in human Hep2 cells using ATP, GTP, UTP, CTP and 1.5 uCi [alpha-32P] NTP as substrate assessed as reduction of total radiolabled transcript after 90 mins by agarose gel electrophoresis
Inhibition of RSV A2 RNA-dependent RNA polymerase expressed in human Hep2 cells using ATP, GTP, UTP, CTP and 1.5 uCi [alpha-32P] NTP as substrate assessed as reduction of total radiolabled transcript after 90 mins by agarose gel electrophoresis
[PMID: 25978965]
Huh-7 CC50
> 50 μM
Compound: 9
Cytotoxicity against human HuH7 cells by MTS assay
Cytotoxicity against human HuH7 cells by MTS assay
[PMID: 20580554]
MT4 CC50
3.8 μM
Compound: 2'd2'FC
Cytotoxicity against human MT4 cells assessed as cell viability after 5 days by Cell-Titer Glo assay
Cytotoxicity against human MT4 cells assessed as cell viability after 5 days by Cell-Titer Glo assay
[PMID: 25978965]
Vero 76 CC50
249 μM
Compound: 2'-fluoro-2'-deoxycytidine
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by neutral red dye based analysis
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by neutral red dye based analysis
[PMID: 36898483]
Vero 76 CC50
277 μM
Compound: 2'-fluoro-2'-deoxycytidine
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by visual analysis
Cytotoxicity against African green monkey Vero 76 cells infected with Measles virus CC by visual analysis
[PMID: 36898483]
In Vitro

2'-Deoxy-2'-fluorocytidine has antiviral activities, showing 50% effective concentrations (EC50) of 61 nM and 31 nM against CCHFV and CCHFV/ZsG in Huh7 cells, respectively. And it shows a 50% cytotoxicity concentration (CC50) of >50.0 μM in Huh7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

245.21

Formula

C9H12FN3O4

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1[C@@H](F)[C@H](N2C(N=C(N)C=C2)=O)O[C@@H]1CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (20.39 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0781 mL 20.3907 mL 40.7814 mL
5 mM 0.8156 mL 4.0781 mL 8.1563 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (407.81 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: 99.98%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.0781 mL 20.3907 mL 40.7814 mL 101.9534 mL
5 mM 0.8156 mL 4.0781 mL 8.1563 mL 20.3907 mL
10 mM 0.4078 mL 2.0391 mL 4.0781 mL 10.1953 mL
15 mM 0.2719 mL 1.3594 mL 2.7188 mL 6.7969 mL
20 mM 0.2039 mL 1.0195 mL 2.0391 mL 5.0977 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Product Name:
2'-Deoxy-2'-fluorocytidine
Cat. No.:
HY-W012009
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