L-Cystine hydrochloride

Name: L-Cystine hydrochloride
Brand: MedChemExpress
SKU: HY-W009356
Rating: 4.5 (1 reviews)

Cat. No.: HY-W009356 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

L-Cystine hydrochloride is an orally active extracellular form of L-Cysteine (HY-Y0337), occurring in proteins of plants and animals. L-Cystine hydrochloride elevates Nrf2 protein expression and activates Nrf2 transcription factor. L-Cystine hydrochloride reduces ROS generation and protects against oxidant- or Doxorubicin (HY-15142A)-induced apoptosis. L-Cystine hydrochloride combined with L-theanine (HY-15121) enhances the production of antigen-specific IgG by increasing glutathione (GSH) levels and T helper 2 (Th2) mediated responses in mice. L-Cystine hydrochloride is promising for research of cystinuria and kidney stones

For research use only. We do not sell to patients.

Custom Peptide Synthesis

L-Cystine hydrochloride Chemical Structure

CAS No. : 34760-60-6

Size	Stock	Quantity
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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50 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of L-Cystine hydrochloride:

L-Cystine monohydrochloride Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

L-Cystine hydrochloride (83 µM or 200 µM, 24 h or 72 h) effectively reverses the growth inhibition of both Sulfasalazine (HY-14655) and Erastin (HY-15763) in Huh6 cells^[1].
L-Cystine hydrochloride ( 0.8 mM, 4 h) induces Nrf2 protein expression elevation in a Keap1-dependent manner in HeLa cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Huh6 and Huh7 cells
Concentration:	83 µM or 200 µM
Incubation Time:	72 h or 24 h
Result:	The degree of growth inhibition by Sulfasalazine (HY-14655) or Erastin (HY-15763) was greater in medium containing a physiological level of 83 μM L-Cystine hydrochloride than in medium containing 200 μM L-Cystine hydrochloride in Huh6 and Huh7 cells.

Western Blot Analysis^[6]

Cell Line:	HeLa cells
Concentration:	0.2 mM
Incubation Time:	4 h
Result:	Was sufficient for producing Nrf2 protein elevation at 0.2 mM in HeLa cells.

Western Blot Analysis^[6]

Cell Line:	HeLa cells
Concentration:	0.8 mM
Incubation Time:	4 h
Result:	Induced Nrf2 in a Keap1-dependent manner in HeLa cells.

In Vivo

L-Cystine hydrochloride (280 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) enhances antigen-specific IgG production partly through augmentation of glutathione (GSH) levels and T helper 2 (Th2)-mediated responses in mice^[2].
L-Cystine hydrochloride (200 mg/kg, p.o., 11 days) administrated with L-theanine (HY-15121) (80 mg/kg, p.o., 11 days) significantly decreases the serum IL-10/IFN-γ ratio 6 hours after DNP-KLH antigenic stimulation, but increases the serum IL-10/IFN-γ ratio 24 hours after DNP-KLH antigenic stimulation in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nine-week-old female BALB/c mice^[2]
Dosage:	140, 280 and 560 mg/kg
Administration:	p.o. administration for 11 days
Result:	Significantly increased IgG and IgM antibody production administrated with L-theanine (HY-15121) after DNP-dextran and DNP-KLH antigenic stimulation in mice.

Molecular Weight

313.22

Formula

C₆H₁₄Cl₂N₂O₄S₂

CAS No.

34760-60-6

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (399.08 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1926 mL	15.9632 mL	31.9264 mL
5 mM	0.6385 mL	3.1926 mL	6.3853 mL
10 mM	0.3193 mL	1.5963 mL	3.1926 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (560 KB)

COA (247 KB) HNMR (246 KB)

Handling Instructions (2659 KB)

References

[1]. Abdullah M, et al. Extracellular Concentration of L-Cystine Determines the Sensitivity to System xc- Inhibitors[J]. Biomol Ther (Seoul). 2022 Mar 1;30(2):184-190. [Content Brief]

[2]. Kurihara S, et al. Enhancement of antigen-specific immunoglobulin G production in mice by co-administration of L-cystine and L-theanine[J]. J Vet Med Sci. 2007 Dec;69(12):1263-70. [Content Brief]

[3]. Dominique Bazin,et al. Cystinuria and cystinosis are usually related to L-cystine: is this really the case for cystinosis? A physicochemical investigation at micrometre and nanometre scale. Comptes Rendus. Chimie, Volume 25 (2022) no. S1, pp. 489-502.

[4]. Greth WE, et al. Cellular accumulation of L-cystine in rat kidney cortex in vivo[J]. J Clin Invest. 1973 Feb;52(2):454-62. [Content Brief]

[5]. Ohtsu I, et al. Uptake of L-cystine via an ABC transporter contributes defense of oxidative stress in the L-cystine export-dependent manner in Escherichia coli. PLoS One. 2015 Apr 2;10(3):e0120619. [Content Brief]

[6]. Dai W, et al. Fresh Medium or L-Cystine as an Effective Nrf2 Inducer for Cytoprotection in Cell Culture[J]. Cells. 2023 Jan 12;12(2):291. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.1926 mL	15.9632 mL	31.9264 mL	79.8161 mL
	5 mM	0.6385 mL	3.1926 mL	6.3853 mL	15.9632 mL
	10 mM	0.3193 mL	1.5963 mL	3.1926 mL	7.9816 mL
	15 mM	0.2128 mL	1.0642 mL	2.1284 mL	5.3211 mL
	20 mM	0.1596 mL	0.7982 mL	1.5963 mL	3.9908 mL
	25 mM	0.1277 mL	0.6385 mL	1.2771 mL	3.1926 mL
	30 mM	0.1064 mL	0.5321 mL	1.0642 mL	2.6605 mL
	40 mM	0.0798 mL	0.3991 mL	0.7982 mL	1.9954 mL
	50 mM	0.0639 mL	0.3193 mL	0.6385 mL	1.5963 mL
	60 mM	0.0532 mL	0.2661 mL	0.5321 mL	1.3303 mL
	80 mM	0.0399 mL	0.1995 mL	0.3991 mL	0.9977 mL
	100 mM	0.0319 mL	0.1596 mL	0.3193 mL	0.7982 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.