3,4-Dihydroxymandelic acid

Name: 3,4-Dihydroxymandelic acid
Brand: MedChemExpress
SKU: HY-113474
Rating: 4.5 (1 reviews)

Cat. No.: HY-113474 Purity: 98.56%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3,4-Dihydroxymandelic acid is a metabolite of norepinephrine.

For research use only. We do not sell to patients.

3,4-Dihydroxymandelic acid Chemical Structure

CAS No. : 775-01-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 62	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 62	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3,4-Dihydroxymandelic acid:

rac 3,4-Dihydroxymandelic Acid-d₃ Get quote
3,4-Dihydroxymandelic acid (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

3,4-Dihydroxymandelic acid is a metabolite of norepinephrine.

IC₅₀ & Target

Human Endogenous Metabolite

Molecular Weight

184.15

Formula

C₈H₈O₅

CAS No.

775-01-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C(O)C1=CC=C(O)C(O)=C1

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (543.04 mM; Need ultrasonic)

DMSO : 83.33 mg/mL (452.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4304 mL	27.1518 mL	54.3036 mL
5 mM	1.0861 mL	5.4304 mL	10.8607 mL
10 mM	0.5430 mL	2.7152 mL	5.4304 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (11.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.56%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (248 KB) HNMR (254 KB) RP-HPLC (211 KB) MS (196 KB)

Handling Instructions (2659 KB)

References

[1]. Sule N, et al. The Norepinephrine Metabolite 3,4-Dihydroxymandelic Acid Is Produced by the Commensal Microbiota and Promotes Chemotaxis and Virulence Gene Expression in Enterohemorrhagic Escherichia coli. Infect Immun. 2017 Sep 20;85(10). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	5.4304 mL	27.1518 mL	54.3036 mL	135.7589 mL
	5 mM	1.0861 mL	5.4304 mL	10.8607 mL	27.1518 mL
	10 mM	0.5430 mL	2.7152 mL	5.4304 mL	13.5759 mL
	15 mM	0.3620 mL	1.8101 mL	3.6202 mL	9.0506 mL
	20 mM	0.2715 mL	1.3576 mL	2.7152 mL	6.7879 mL
	25 mM	0.2172 mL	1.0861 mL	2.1721 mL	5.4304 mL
	30 mM	0.1810 mL	0.9051 mL	1.8101 mL	4.5253 mL
	40 mM	0.1358 mL	0.6788 mL	1.3576 mL	3.3940 mL
	50 mM	0.1086 mL	0.5430 mL	1.0861 mL	2.7152 mL
	60 mM	0.0905 mL	0.4525 mL	0.9051 mL	2.2626 mL
	80 mM	0.0679 mL	0.3394 mL	0.6788 mL	1.6970 mL
	100 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3576 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.