3-Azido-7-hydroxycoumarin

Cat. No.: HY-W540978 Purity: 97.40%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

3-Azido-7-hydroxycoumarin is a click chemistry reagent containing an azide group. Under the catalysis of Cu(II), it can undergo an azide-alkyne cycloaddition (CuAAC) click reaction with 3-butyn-1-ol and emit a fluorescent signal.

For research use only. We do not sell to patients.

3-Azido-7-hydroxycoumarin Chemical Structure

CAS No. : 817638-68-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote	Estimated Time of Arrival: December 31
100 mg	Get quote	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Purity & Documentation
References
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Description

Molecular Weight

203.15

Formula

C₉H₅N₃O₃

CAS No.

817638-68-9

Appearance

Solid

Color

Yellow to brown

SMILES

OC1=CC(OC(C(N=[N+]=[N-])=C2)=O)=C2C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (246.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9225 mL	24.6124 mL	49.2247 mL
5 mM	0.9845 mL	4.9225 mL	9.8449 mL
10 mM	0.4922 mL	2.4612 mL	4.9225 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.40%

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Data Sheet (268 KB) SDS (584 KB)

COA (246 KB) HNMR (272 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. You H, et al. Ultrasensitive and Specific Phage@DNAzyme Probe-Triggered Fluorescent Click Chemistry for On-Site Detection of Foodborne Pathogens Using a Smartphone. Anal Chem. 2023 Aug 1;95(30):11211-11218. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.9225 mL	24.6124 mL	49.2247 mL	123.0618 mL
	5 mM	0.9845 mL	4.9225 mL	9.8449 mL	24.6124 mL
	10 mM	0.4922 mL	2.4612 mL	4.9225 mL	12.3062 mL
	15 mM	0.3282 mL	1.6408 mL	3.2816 mL	8.2041 mL
	20 mM	0.2461 mL	1.2306 mL	2.4612 mL	6.1531 mL
	25 mM	0.1969 mL	0.9845 mL	1.9690 mL	4.9225 mL
	30 mM	0.1641 mL	0.8204 mL	1.6408 mL	4.1021 mL
	40 mM	0.1231 mL	0.6153 mL	1.2306 mL	3.0765 mL
	50 mM	0.0984 mL	0.4922 mL	0.9845 mL	2.4612 mL
	60 mM	0.0820 mL	0.4102 mL	0.8204 mL	2.0510 mL
	80 mM	0.0615 mL	0.3077 mL	0.6153 mL	1.5383 mL
	100 mM	0.0492 mL	0.2461 mL	0.4922 mL	1.2306 mL