360A

Name: 360A
Brand: MedChemExpress
SKU: HY-15595
Rating: 5.0 (2 reviews)

Cat. No.: HY-15595: FAQs
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360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC₅₀ of 300 nM for telomerase in TRAP-G4 assay.

For research use only. We do not sell to patients.

360A Chemical Structure

CAS No. : 794458-56-3

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Based on 2 publication(s) in Google Scholar

Other Forms of 360A:

360A iodide In-stock

2 Publications Citing Use of MCE 360A

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Description

360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC₅₀ of 300 nM for telomerase in TRAP-G4 assay.

IC₅₀ & Target

IC50: 300 nM (Telomerase)^[1]
G-quadruplex^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	45 nM Compound: 360A	Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	52 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	5600 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	63 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	8400 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay	[PMID: 17954919]

In Vitro

360A inhibits telomerase activity and stabilizes G-quadruplex, with an IC₅₀ of 300 nM for telomerase in TRAP-G4 assay. 360A reduces the viability of glioma cell lines, such as T98G, CB193, U118-MG, SAOS-2 and Primary astrocytes, with IC₅₀s of 4.8 ± 1.1 μM, 3.9 ± 0.4 μM, 8.4 ± 0.5 μM, >15 μM and 17.4 ± 1.2 μM, respectively^[1]. 360A causes Rad51-dependent telomere aberrations preferentially involving the lagging strand telomeres, including telomere losses or telomere doublets, and induces DNA-PKcs-dependent sister telomere fusions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

449.50

Formula

C₂₇H₂₃N₅O₂

CAS No.

794458-56-3

SMILES

O=C(C1=CC=CC(C(NC2=C[N+](C)=C3C=CC=CC3=C2)=O)=N1)NC4=C[N+](C)=C5C=CC=CC5=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief]

[2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]

Cell Assay
^[1]

The cell proliferation reagent WST-1 assay is performed. In brief, cells are seeded at various densities, depending on cell type (0.25-4 × 10³ cells/well in 100 μL complete medium), in 96-well culture plates and treated with various concentrations (0.1-20 μM) of 360A or the corresponding concentrations of DMSO (control wells) for 3 or 7 days at 37°C in an atmosphere containing 5% CO₂. For 7-day assays, the medium is changed on day 3. Experiments are performed in triplicate^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief]

[2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]

360A Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

360A794458-56-3G-quadruplexTelomeraseInhibitorinhibitorinhibit

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360A

Customer Review

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360A Related Antibodies

2 Publications Citing Use of MCE 360A

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360A Related Antibodies

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