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  6. 3x DYKDDDDK Tag TFA

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance .

For research use only. We do not sell to patients.

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3x DYKDDDDK Tag TFA Chemical Structure

3x DYKDDDDK Tag TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 75 In-stock
5 mg USD 220 In-stock
10 mg USD 360 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of 3x DYKDDDDK Tag TFA:

3x DYKDDDDK Tag TFA Related Antibodies

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Description

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance [1][2].

In Vitro

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3x DYKDDDDK Tag TFA Related Antibodies
Molecular Weight

3116.90

Formula

C125H177F3N30O60
Appearance

Solid

Color

White to off-white

Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (32.08 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3208 mL 1.6042 mL 3.2083 mL
5 mM 0.0642 mL 0.3208 mL 0.6417 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.3208 mL 1.6042 mL 3.2083 mL 8.0208 mL
5 mM 0.0642 mL 0.3208 mL 0.6417 mL 1.6042 mL
10 mM 0.0321 mL 0.1604 mL 0.3208 mL 0.8021 mL
15 mM 0.0214 mL 0.1069 mL 0.2139 mL 0.5347 mL
20 mM 0.0160 mL 0.0802 mL 0.1604 mL 0.4010 mL
25 mM 0.0128 mL 0.0642 mL 0.1283 mL 0.3208 mL
30 mM 0.0107 mL 0.0535 mL 0.1069 mL 0.2674 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3x DYKDDDDK Tag TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3x DYKDDDDK TagOthersInhibitorinhibitorinhibit

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