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  4. 4-Di-1-ASP

4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λex /λem = 475/606 nm) .

For research use only. We do not sell to patients.

4-Di-1-ASP Chemical Structure

4-Di-1-ASP Chemical Structure

CAS No. : 959-81-9

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Based on 1 publication(s) in Google Scholar

4-Di-1-ASP Related Antibodies

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Description

4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λex /λem = 475/606 nm) [1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1.Dissolve the dye in water and configure a mother liquor at a concentration of 2 mM.
2.Place the prepared cell sections in an incubator at 35°C, add the prepared dye solution to a final concentration of 1 μM and incubate with the stain for 10 min.
3.The prepared stained cell sections are placed on a microscope slide, cover with a coverslip and observe using a fluorescent confocal microscope with a maximum excitation wavelength of 475 nm and a maximum emission wavelength of 606 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Di-1-ASP Related Antibodies
Molecular Weight

366.24

Formula

C16H19IN2
CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

521
Excitation (Ex)

488
SMILES

C[N+]1=CC=C(/C=C/C2=CC=C(N(C)C)C=C2)C=C1.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 43.75 mg/mL (119.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7304 mL 13.6523 mL 27.3045 mL
5 mM 0.5461 mL 2.7304 mL 5.4609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.95%

SDS (252 KB)

COA (245 KB) HNMR (240 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7305 mL 13.6523 mL 27.3045 mL 68.2613 mL
5 mM 0.5461 mL 2.7305 mL 5.4609 mL 13.6523 mL
10 mM 0.2730 mL 1.3652 mL 2.7305 mL 6.8261 mL
15 mM 0.1820 mL 0.9102 mL 1.8203 mL 4.5508 mL
20 mM 0.1365 mL 0.6826 mL 1.3652 mL 3.4131 mL
25 mM 0.1092 mL 0.5461 mL 1.0922 mL 2.7305 mL
30 mM 0.0910 mL 0.4551 mL 0.9102 mL 2.2754 mL
40 mM 0.0683 mL 0.3413 mL 0.6826 mL 1.7065 mL
50 mM 0.0546 mL 0.2730 mL 0.5461 mL 1.3652 mL
60 mM 0.0455 mL 0.2275 mL 0.4551 mL 1.1377 mL
80 mM 0.0341 mL 0.1707 mL 0.3413 mL 0.8533 mL
100 mM 0.0273 mL 0.1365 mL 0.2730 mL 0.6826 mL
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4-Di-1-ASP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Di-1-ASP959-81-9Fluorescent Dyestyryl dyefluorescent dyelive cell stainInhibitorinhibitorinhibit

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