4′-Hydroxy Nitazene

Cat. No.: HY-164746 Purity: 99.6%: FAQs
Data Sheet SDS COA Handling Instructions

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4′-Hydroxy Nitazene is the universal metabolite of the nitazene analogs. 4′-Hydroxy Nitazene is a metabolite of isotonitazene.

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4′-Hydroxy Nitazene Chemical Structure

CAS No. : 94758-81-3

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote
10 mg	Get quote

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Description

4′-Hydroxy Nitazene is the universal metabolite of the nitazene analogs. 4′-Hydroxy Nitazene is a metabolite of isotonitazene^[1]^[2].

Molecular Weight

368.43

Formula

C₂₀H₂₄N₄O₃

CAS No.

94758-81-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+](C1=CC(N=C(CC2=CC=C(C=C2)O)N3CCN(CC)CC)=C3C=C1)[O-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMF:PSB 1:1 : 25 mg/mL (67.86 mM; adjust pH to 7.2)

Ethanol : 10 mg/mL (27.14 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7142 mL	13.5711 mL	27.1422 mL
5 mM	0.5428 mL	2.7142 mL	5.4284 mL
10 mM	0.2714 mL	1.3571 mL	2.7142 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.6%

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Data Sheet (274 KB) SDS (393 KB)

COA (252 KB)

Handling Instructions (2659 KB)

References

[1]. Krotulski AJ, et al. Isotonitazene Quantitation and Metabolite Discovery in Authentic Forensic Casework. J Anal Toxicol. 2020 Jul 31;44(6):521-530. [Content Brief]

[2]. Walton SE, et al. A Forward-Thinking Approach to Addressing the New Synthetic Opioid 2-Benzylbenzimidazole Nitazene Analogs by Liquid Chromatography-Tandem Quadrupole Mass Spectrometry (LC-QQQ-MS). J Anal Toxicol. 2022 Mar 21;46(3):221-231. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMF:PSB 1:1	1 mM	2.7142 mL	13.5711 mL	27.1422 mL	67.8555 mL
	5 mM	0.5428 mL	2.7142 mL	5.4284 mL	13.5711 mL
	10 mM	0.2714 mL	1.3571 mL	2.7142 mL	6.7855 mL
	15 mM	0.1809 mL	0.9047 mL	1.8095 mL	4.5237 mL
	20 mM	0.1357 mL	0.6786 mL	1.3571 mL	3.3928 mL
	25 mM	0.1086 mL	0.5428 mL	1.0857 mL	2.7142 mL
DMF:PSB 1:1	30 mM	0.0905 mL	0.4524 mL	0.9047 mL	2.2618 mL
	40 mM	0.0679 mL	0.3393 mL	0.6786 mL	1.6964 mL
	50 mM	0.0543 mL	0.2714 mL	0.5428 mL	1.3571 mL
	60 mM	0.0452 mL	0.2262 mL	0.4524 mL	1.1309 mL