6-NBDG 

6-NBDG is a fluorescent glucose analogue, it can be used for fluorescence imaging and monitoring glucose transport and uptake. 6-NBDG can be used as a fluorescent probe for detecting macrophage-rich atherosclerotic plaques^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Incubate sample with RPMI (without glucose) containing a final concentration of 200 μM 6-NBDG.
2. Use a curved forcep to embeds sample into the mixture, and stretches the samplet through a drop of agarose (40°C, 20 μL) places on a pre-heated (40°C) glass slide.
3. Gently place another preheated glass slide on the top of sample, so that the whole sample embeds into the agarose-6-NBDG mixture.
4. Take confocal images with a confocal laser scanning microscope (Ex: 471 nm, Em: 500–600 nm)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

342.26

C₁₂H₁₄N₄O₈

108708-22-1

Solid

Orange to red

542

488

O=C[C@@H]([C@H]([C@@H]([C@@H](CNC1=CC=C([N+]([O-])=O)C2=NON=C21)O)O)O)O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Hansen TV, et al. Glucose Absorption by the Bacillary Band of Trichuris muris. PLoS Negl Trop Dis. 2016 Sep 2;10(9):e0004971.  [Content Brief]

  [2]. Zaman RT, et al. Fiber-optic system for dual-modality imaging of glucose probes 18F-FDG and 6-NBDG in atherosclerotic plaques. PLoS One. 2014 Sep 18;9(9):e108108.  [Content Brief]