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  3. Fluorescent Dye
  4. 7-Hydroxycoumarin-4-acetic acid

7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λmax=326 nm).

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7-Hydroxycoumarin-4-acetic acid Chemical Structure

7-Hydroxycoumarin-4-acetic acid Chemical Structure

CAS No. : 6950-82-9

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7-Hydroxycoumarin-4-acetic acid Related Antibodies

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Description

7-Hydroxycoumarin-4-acetic acid, a pH-indicator dye, is covalently bound to bovine serum albumin (BSA) at the positions of reactive amino groups (λmax=326 nm)[1].

Cellular Effect
Cell Line Type Value Description References
B16-F10 IC50
> 10 μM
Compound: HCAA
Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against mouse B16-F10 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
[PMID: 33956447]
HepG2 IC50
> 10 μM
Compound: HCAA
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
[PMID: 33956447]
HT-29 IC50
> 10 μM
Compound: HCAA
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
[PMID: 33956447]
Molecular Weight

220.18

Formula

C11H8O5
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

450
Excitation (Ex)

415
SMILES

O=C(O)CC(C1=CC=C(O)C=C1O2)=CC2=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
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7-Hydroxycoumarin-4-acetic acid Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Hydroxycoumarin-4-acetic acid6950-82-9Fluorescent DyeBSAbovine serum albuminpH-indicatorInhibitorinhibitorinhibit

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7-Hydroxycoumarin-4-acetic acid
Cat. No.:
HY-D0112
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