2. Metabolic Enzyme/Protease
  3. Drug Metabolite
  4. 7-Hydroxymethotrexate

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis.

For research use only. We do not sell to patients.

7-Hydroxymethotrexate Chemical Structure

7-Hydroxymethotrexate Chemical Structure

CAS No. : 5939-37-7

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Other Forms of 7-Hydroxymethotrexate:

7-Hydroxymethotrexate Related Antibodies

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Description

7-Hydroxymethotrexate is a major metabolite of Methotrexate (MTX; HY-14519). Methotrexate, an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis[1][2].

In Vitro

7-Hydroxymethotrexate (7-OHMTX) has the affinity of DHFR markedly lower (>100-fold) than for Methotrexate (MTX) [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Hydroxymethotrexate Related Antibodies
In Vivo

7-Hydroxymethotrexate (4 mg/kg; IV) has a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min mg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (270-440 g)[1]
Dosage: 4 mg/kg (Pharmacokinetic Analysis)
Administration: IV
Result: Had a terminal half-life of 97.2 min and a total clearance value of 9.6 mL/min•mg.
Molecular Weight

470.44

Formula

C20H22N8O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(N(CC2=NC3=C(N)N=C(N)N=C3NC2=O)C)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation

Purity: 98.2%

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COA (246 KB)

Handling Instructions (2659 KB)
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7-Hydroxymethotrexate Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Hydroxymethotrexate5939-37-7Drug MetaboliteMethotrexateMTXantimetaboliteantifolateenzymedihydrofolatereductasetetrahydrofolateDNAsynthesisInhibitorinhibitorinhibit

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