7-Methoxycoumarin-3-carboxylic acid

Name: 7-Methoxycoumarin-3-carboxylic acid
Brand: MedChemExpress
SKU: HY-W039519
Rating: 4.5 (1 reviews)

Cat. No.: HY-W039519 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

7-Methoxycoumarin-3-carboxylic acid is a fluorescent dye with an excitation peak at 355 nm and an emission peak at 405 nm. 7-Methoxycoumarin-3-carboxylic acid can be used to label peptide.

For research use only. We do not sell to patients.

7-Methoxycoumarin-3-carboxylic acid Chemical Structure

CAS No. : 20300-59-8

Size	Stock	Quantity
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

7-Methoxycoumarin-3-carboxylic acid is a fluorescent dye with an excitation peak at 355 nm and an emission peak at 405 nm. 7-Methoxycoumarin-3-carboxylic acid can be used to label peptide^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Oocyte	IC₅₀	168 μM Compound: 7-OMe-Coum-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
SiHa	IC₅₀	2.3 μM Compound: 1	Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting	[PMID: 24095010]
SiHa	EC₅₀	9.8 μM Compound: 1	Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay	[PMID: 24095010]

In Vitro

7-Methoxycoumarin-3-carboxylic acid (compound 5) (100 μM; 72 h) shows non-toxic for K562, Daudi, RPMI-8226, and Jurkat cells with IC₅₀s of >100, >100, >100, >100 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

220.18

Formula

C₁₁H₈O₅

CAS No.

20300-59-8

Appearance

Solid

Color

White to off-white

Emission (Em)

450

Excitation (Ex)

345

SMILES

O=C(C1=CC2=CC=C(OC)C=C2OC1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 8 mg/mL (36.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5417 mL	22.7087 mL	45.4174 mL
5 mM	0.9083 mL	4.5417 mL	9.0835 mL
10 mM	0.4542 mL	2.2709 mL	4.5417 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (248 KB) HNMR (117 KB) RP-HPLC (205 KB)

Handling Instructions (2659 KB)

References

[1]. Wiemer AJ, et al. Evaluation of a 7-Methoxycoumarin-3-carboxylic Acid Ester Derivative as a Fluorescent, Cell-Cleavable, Phosphonate Protecting Group. Chembiochem. 2016 Jan 1;17(1):52-5. [Content Brief]

[2]. Mohamed-Anis Alouini, et al. Interaction of fluorescently labeled triethyleneglycol and peptide derivatives with β-cyclodextrin. Chemphyschem. 2014 Feb 24;15(3):444-57. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5417 mL	22.7087 mL	45.4174 mL	113.5435 mL
	5 mM	0.9083 mL	4.5417 mL	9.0835 mL	22.7087 mL
	10 mM	0.4542 mL	2.2709 mL	4.5417 mL	11.3543 mL
	15 mM	0.3028 mL	1.5139 mL	3.0278 mL	7.5696 mL
	20 mM	0.2271 mL	1.1354 mL	2.2709 mL	5.6772 mL
	25 mM	0.1817 mL	0.9083 mL	1.8167 mL	4.5417 mL
	30 mM	0.1514 mL	0.7570 mL	1.5139 mL	3.7848 mL