Calyculin A (Synonyms: (-)-Calyculin A)

Cat. No.: HY-18983: FAQs
Data Sheet SDS COA Handling Instructions

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Calyculin A ((-)-Calyculin A) is a potent and cell-permeable protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) inhibitor with IC₅₀s of 2 nM and 0.5-1 nM, respectively.

For research use only. We do not sell to patients.

Calyculin A Chemical Structure

CAS No. : 101932-71-2

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14 Publications Citing Use of MCE Calyculin A

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Description

Calyculin A ((-)-Calyculin A) is a potent and cell-permeable protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) inhibitor with IC₅₀s of 2 nM and 0.5-1 nM, respectively^[1].

IC₅₀ & Target

IC50: 2 nM (PP1); 0.5-1 nM (PP2A)^[1]

In Vitro

Calyculin A (1-10 nM; 24 hours) induces cytotoxicity in MG63 cells in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	Human osteosarcoma MG63 cells
Concentration:	0, 1, 2, 5, 10 nM
Incubation Time:	24 hours
Result:	Treatment with 0.1 nM for 24 h had a minimal effect on MG63 cell survival. Cell rounding and shrinking were obvious in the cultures treated for 24 h with 5 nM. The level of the cell viability treated with 10 nM was 27% that of the control cultures.

Molecular Weight

1009.17

Formula

C₅₀H₈₁N₄O₁₅P

CAS No.

101932-71-2

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O[C@@H]([C@H]([C@@H](N(C)C)COC)O)C(NCC[C@@H](C1=NC(/C=C/C[C@@H]([C@@H](C)[C@H](O)C2)O[C@@]32C(C)(C)[C@@H](OP(O)(O)=O)[C@@H]([C@@H](OC)C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C(C)/C(C)=C/C=C/C(C)=C\C#N)O3)=CO1)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Purity & Documentation

Purity: 99.67%

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Data Sheet (271 KB) SDS (420 KB)

COA (250 KB) RP-HPLC (108 KB)

Handling Instructions (2659 KB)

References

[1]. Ishihara H, et al. Calyculin A and okadaic acid: inhibitors of protein phosphatase activity. Biochem Biophys Res Commun. 1989 Mar 31;159(3):871-7. [Content Brief]

[2]. Hiroaki Tanaka,et al. Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells. Int J Oncol. 2007 Aug;31(2):389-96. [Content Brief]

Calyculin A Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calyculin A101932-71-2(-)-Calyculin APhosphataseInhibitorinhibitorinhibit

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