DHBS (Synonyms: DCHBS)

Cat. No.: HY-15914 Purity: 99.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H₂O₂) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems.

For research use only. We do not sell to patients.

DHBS Chemical Structure

CAS No. : 54970-72-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g	USD 60	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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This product is a controlled substance and not for sale in your territory.

Other Forms of DHBS:

3,5-Dichloro-2-hydroxybenzenesulfonic acid Get quote

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References
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Description

In Vitro

DHBS (750 μM, 5 min) significantly enhances nitroblue tetrazolium (NBT) induced O₂ reduction in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

265.05

Formula

C₆H₃Cl₂NaO₄S

CAS No.

54970-72-8

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC(Cl)=CC(Cl)=C1O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (377.29 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7729 mL	18.8644 mL	37.7287 mL
5 mM	0.7546 mL	3.7729 mL	7.5457 mL
10 mM	0.3773 mL	1.8864 mL	3.7729 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (94.32 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (253 KB) RP-HPLC (80 KB)

Handling Instructions (2659 KB)

References

[1]. Tarasek D, et al. In vitro oxidation of hispidin and gallic acid by horseradish peroxidase[J]. J Biomol Struct Dyn. 2023 Apr;41(6):2321-2325. [Content Brief]

[2]. Aizenman E. Modulation of N-methyl-D-aspartate receptors by hydroxyl radicals in rat cortical neurons in vitro[J]. Neurosci Lett. 1995 Apr 7;189(1):57-9. [Content Brief]

[3]. Colliss JS, et al. The analysis of serum urate utilizing immobilized uricase[J]. Appl Biochem Biotechnol. 1981 Mar;6(1):15-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.7729 mL	18.8644 mL	37.7287 mL	94.3218 mL
	5 mM	0.7546 mL	3.7729 mL	7.5457 mL	18.8644 mL
	10 mM	0.3773 mL	1.8864 mL	3.7729 mL	9.4322 mL
	15 mM	0.2515 mL	1.2576 mL	2.5152 mL	6.2881 mL
	20 mM	0.1886 mL	0.9432 mL	1.8864 mL	4.7161 mL
	25 mM	0.1509 mL	0.7546 mL	1.5091 mL	3.7729 mL
	30 mM	0.1258 mL	0.6288 mL	1.2576 mL	3.1441 mL
	40 mM	0.0943 mL	0.4716 mL	0.9432 mL	2.3580 mL
	50 mM	0.0755 mL	0.3773 mL	0.7546 mL	1.8864 mL
	60 mM	0.0629 mL	0.3144 mL	0.6288 mL	1.5720 mL
	80 mM	0.0472 mL	0.2358 mL	0.4716 mL	1.1790 mL
	100 mM	0.0377 mL	0.1886 mL	0.3773 mL	0.9432 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

DHBS Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DHBS54970-72-8DCHBSFluorescent DyeperoxidaseO₂hydrogen peroxideautomated systemsInhibitorinhibitorinhibit

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