2. Membrane Transporter/Ion Channel
  3. Chloride Channel
  4. H100

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

For research use only. We do not sell to patients.

H100 Chemical Structure

H100 Chemical Structure

CAS No. : 643727-55-3

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

H100 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

IC50 & Target

Cl- transport[1]
In Vitro

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H100 Related Antibodies
Clinical Trial
Molecular Weight

388.39

Formula

C18H16N2O6S
CAS No.
SMILES

O=C(O)C1=CC(N2C=CC=C2)=C(OC3=CC=C(OC)C=C3)C(S(=O)(N)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Cell Assay
[1]

35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

H100 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H100643727-55-3H 100H-100Chloride ChannelCl− ChannelsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
H100
Cat. No.:
HY-100322
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.