2. Membrane Transporter/Ion Channel
  3. Chloride Channel
  4. H100

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

For research use only. We do not sell to patients.

H100 Chemical Structure

H100 Chemical Structure

CAS No. : 643727-55-3

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H100 Related Antibodies

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Description

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.

IC50 & Target

Cl- transport[1]
In Vitro

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

H100 Related Antibodies
Clinical Trial
Molecular Weight

388.39

Formula

C18H16N2O6S
CAS No.
SMILES

O=C(O)C1=CC(N2C=CC=C2)=C(OC3=CC=C(OC)C=C3)C(S(=O)(N)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Cell Assay
[1]

35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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H100 Related Classifications

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H100643727-55-3H 100H-100Chloride ChannelCl− ChannelsInhibitorinhibitorinhibit

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