Hexa-His (Synonyms: 6X His Tag)

Name: Hexa-His
Brand: MedChemExpress
SKU: HY-P0294
Rating: 5.0 (1 reviews)

Cat. No.: HY-P0294 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni²⁺) or cobalt (Co²⁺), making it useful for protein purification.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Hexa-His Chemical Structure

CAS No. : 64134-30-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hexa-His:

Hexa-His TFA Get quote

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Hexa-His for Protein Purification and Protein Analysis^[2]
(1) Construct a plasmid containing the target gene and Hexa-His tag, and introduce it into E. coli.
(2) Select positive clones and grow them in LB medium until the OD600 is about 0.6-0.8, then add IPTG (HY-15921, isopropyl β-D-thiogalactoside) as an inducer and continue to incubate for a few hours to induce the expression of the target protein.
(3) Collect the bacterial cells, resuspend them in lysis buffer, and sonicate to lyse the cells, then centrifuge to remove debris and obtain the supernatant.
(4) Prepare the Ni-NTA affinity chromatography column and slowly pass the lysate supernatant through the Ni-NTA column to bind the CTB protein with the Hexa-His tag to the column.
(5) After protein adsorption, wash the resin with 10 column volumes of 60 mM imidazole binding buffer. Elute the target gene with the same solution containing 1 M imidazole using 5 column volumes. Collect the protein and analyze it by 15% SDS-PAGE.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

840.85

Formula

C₃₆H₄₄N₁₈O₇

CAS No.

64134-30-1

Appearance

Solid

Color

White to off-white

Sequence

His-His-His-His-His-His

Sequence Shortening

HHHHHH

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (59.46 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1893 mL	5.9464 mL	11.8927 mL
5 mM	0.2379 mL	1.1893 mL	2.3785 mL
10 mM	0.1189 mL	0.5946 mL	1.1893 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (118.93 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (249 KB) RP-HPLC (246 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Watly J, et al. Insight into the coordination and the binding sites of Cu(2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53(13):6675-83. [Content Brief]

[2]. Ana Paula de Mattos Arêas, et al. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli.Protein Expr Purif.2002 Aug;25(3):481-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.1893 mL	5.9464 mL	11.8927 mL	29.7318 mL
	5 mM	0.2379 mL	1.1893 mL	2.3785 mL	5.9464 mL
	10 mM	0.1189 mL	0.5946 mL	1.1893 mL	2.9732 mL
	15 mM	0.0793 mL	0.3964 mL	0.7928 mL	1.9821 mL
	20 mM	0.0595 mL	0.2973 mL	0.5946 mL	1.4866 mL
	25 mM	0.0476 mL	0.2379 mL	0.4757 mL	1.1893 mL
	30 mM	0.0396 mL	0.1982 mL	0.3964 mL	0.9911 mL
	40 mM	0.0297 mL	0.1487 mL	0.2973 mL	0.7433 mL
	50 mM	0.0238 mL	0.1189 mL	0.2379 mL	0.5946 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Hexa-His Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hexa-His64134-30-16X His TagOthersseparationpurificationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Hexa-His (Synonyms: 6X His Tag)

Customer Review

Other Forms of Hexa-His:

Hexa-His Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Hexa-His Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Hexa-His Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report