Hoechst 33258 analog

Name: Hoechst 33258 analog
Brand: MedChemExpress
SKU: HY-15623
Rating: 4.5 (1 reviews)

Cat. No.: HY-15623 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Hoechst 33258 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.

For research use only. We do not sell to patients.

Hoechst 33258 analog Chemical Structure

CAS No. : 258843-62-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 472	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 472	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 560	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1000	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2050	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hoechst 33258 analog:

1 Publications Citing Use of MCE Hoechst 33258 analog

•Saudi J Biol Sci. 2021 May 8.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

General Protocol
Preparation of Hoechst working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of in 5 mL DMSO
Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Hoechst working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution.
Note: Please adjust the concentration of Hoechst working solution according to the actual situation.
1.Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions
1. Please adjust the concentration of Hoechst working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

510.59

Formula

C₂₉H₃₀N₆O₃

CAS No.

258843-62-8

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

451

Excitation (Ex)

356

SMILES

O=C(O)CCCOC1=CC=CC(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 51 mg/mL (99.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9585 mL	9.7926 mL	19.5852 mL
5 mM	0.3917 mL	1.9585 mL	3.9170 mL
10 mM	0.1959 mL	0.9793 mL	1.9585 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (243 KB) HNMR (205 KB) LCMS (400 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: Hoechst 33258 analog can be used to stain DNA for detecting apoptosis.
Method: For cell staining.
1. 48 h after transfection, cell slides are prepared and fixed in 4% paraformaldehyde.
2. Stain cells with Hoechst 33258 analog (30 min; dark) solution.
3. Use a fluorescence microscope (Nikon Instruments Inc., Tokyo, Japan) for image.

References

[1]. a b c "Hoechst Stains". Invitrogren (Molecular Probes).

[2]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. [Content Brief]

[3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9585 mL	9.7926 mL	19.5852 mL	48.9630 mL
	5 mM	0.3917 mL	1.9585 mL	3.9170 mL	9.7926 mL
	10 mM	0.1959 mL	0.9793 mL	1.9585 mL	4.8963 mL
	15 mM	0.1306 mL	0.6528 mL	1.3057 mL	3.2642 mL
	20 mM	0.0979 mL	0.4896 mL	0.9793 mL	2.4481 mL
	25 mM	0.0783 mL	0.3917 mL	0.7834 mL	1.9585 mL
	30 mM	0.0653 mL	0.3264 mL	0.6528 mL	1.6321 mL
	40 mM	0.0490 mL	0.2448 mL	0.4896 mL	1.2241 mL
	50 mM	0.0392 mL	0.1959 mL	0.3917 mL	0.9793 mL
	60 mM	0.0326 mL	0.1632 mL	0.3264 mL	0.8160 mL
	80 mM	0.0245 mL	0.1224 mL	0.2448 mL	0.6120 mL