L162389

Cat. No.: HY-101618: FAQs
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L162389 is a potent antagonist of angiotensin AT1 receptor with K_i of 28 nM.

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L162389 Chemical Structure

CAS No. : 169281-53-2

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AT1 Receptor AT2 Receptor

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Description	L162389 is a potent antagonist of angiotensin AT1 receptor with K_i of 28 nM.
IC₅₀ & Target	Ki: 28 nM (angiotensin AT1 receptor)^[1]
In Vitro	L-162,389 stimulates phosphatidylinositol turnover, albeit only to a small percentage of the angiotensin response. L-162,389 acts as angiotensin antagonist with IC₅₀ value of 105 nM^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. L162389 Related Antibodies
Molecular Weight	562.72
Formula	C₃₁H₃₈N₄O₄S
CAS No.	169281-53-2
SMILES	O=C(OCCCC)NS(=O)(C1=CC=C(CCC)C=C1C2=CC=C(CN3C(CC)=NC4=C(C)C=C(C)N=C43)C=C2)=O
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (273 KB) Handling Instructions (2659 KB)
References	[1]. Perlman S, et al. Dual agonistic and antagonistic property of nonpeptide angiotensin AT1 ligands: susceptibility to receptor mutations. Mol Pharmacol. 1997 Feb;51(2):301-11. [Content Brief]

Kinase Assay
^[1]

Monoiodinated ¹²⁵I-[Sar1,Leu8]angiotensin II is prepared by the Iodo-Gen method. One day after transfection and 24 hr before the binding experiments, the transfected cells are transferred to 6-, 12-, or 24-well culture plates, with 0.15-9 × 10⁵ cells/well, with a goal of total binding of 5-10% of the radiolabeled peptide. The cells are washed twice with buffer (25 mM Tris, 5 mM MgCl₂, 140 mM NaCl, pH 7.4) before and after the binding. The binding is carried out for 24 hr at 4°C with 50 pm ¹²⁵I-[Sar1,Leu8]angiotensin II and variable amounts of unlabeled nonpeptide or peptide ligands in 0.5-1 mL of a 25 mM Tris buffer containing 5 mm MgCl₂, pH 7.4. The binding data are analyzed by computerized nonlinear regression analysis using InPlot 4.0.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Perlman S, et al. Dual agonistic and antagonistic property of nonpeptide angiotensin AT1 ligands: susceptibility to receptor mutations. Mol Pharmacol. 1997 Feb;51(2):301-11. [Content Brief]

L162389 Related Classifications

Cardiovascular Disease

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L162389169281-53-2L 162389L-162389Angiotensin ReceptorInhibitorinhibitorinhibit

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